Anti-PCSK9 Antibody (N-term)

Product Name

Anti-PCSK9 Antibody (N-term)

View all Proprotein Convertase 9/PCSK9 Antibodies

SKU/Catalog Number

A00085-3

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-PCSK9 Antibody (N-term) (Catalog # A00085-3). Tested in Flow Cytometry, IHC-P, WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-PCSK9 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00085-3)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This PCSK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-183 amino acids from the N-terminal region of human PCSK9.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00085-3 is reactive to Pcsk9 in Human

Applications

A00085-3 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

74.823kDa

Background of Proprotein Convertase 9/PCSK9

PCSK9 is a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. The protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of lysate from SW480 cell line, using PCSK9 Antibody (N-term). A00085-3 was diluted at 1:2000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Click image to see more details

Western blot analysis of lysate from SW480 cell line, using PCSK9 Antibody (N-term). A00085-3 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Click image to see more details

Western blot analysis of lysate from Hela cell line, using PCSK9 Antibody (N-term). A00085-3 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Click image to see more details

Western blot analysis of PCSK9 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). PCSK9 (arrow) was detected using the purified Pab.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using PCSK9 Antibody (N-term). A00085-3 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Click image to see more details

PCSK9 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PCSK9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Click image to see more details

Flow cytometric analysis of A431 cells using PCSK9 Antibody (N-term) (green, Cat#A00085-3) compared to an isotype control of rabbit IgG(blue). A00085-3 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Gene/Protein Information For Pcsk9 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; FHCL3; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9 Pcsk9|AI415265, AI747682, FH3, HCHOLA3, Na, Narc1, PC9|proprotein convertase subtilisin/kexin type 9|proprotein convertase subtilisin/kexin type 9|NARC-1|convertase subtilisin|neural apoptosis regulated convertase 1|proprotein convertase 9|proprotein convertase PC9|subtilisin/kexin-like protease PC9

For more info on Pcsk9, check out the Pcsk9 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Pcsk9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Pcsk9 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis