Product Info Summary
SKU: | A01906-2 |
---|---|
Size: | 100μl |
Reactive Species: | Bovine, Human, Mouse, Pig, Rat |
Host: | Mouse |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-PDH-E1 alpha PDHA1 Monoclonal Antibody
View all Pyruvate Dehydrogenase E1-alpha subunit Antibodies
SKU/Catalog Number
A01906-2
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PDH-E1 alpha PDHA1 Monoclonal Antibody catalog # A01906-2. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDH-E1 alpha PDHA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01906-2)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PDHA1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01906-2 is reactive to PDHA1 in Bovine, Human, Mouse, Pig, Rat
Applications
A01906-2 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.296kDa
Background of Pyruvate Dehydrogenase E1-alpha subunit
CaM Kinase IV (also known as CAM kinase-GR and CaMK IV) is a calcium/ calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. This kinase may be involved in the transcriptional regulation of microtubule dynamics. In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. CaMK IV may also be involved in spermatogenesis and may play a role in the consolidation/ retention of hippocampus-dependent long-term memory. CaMK IV must be phosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2. In addition autophosphorylation of the N-terminus is required for full activation. Autophosphorylation of Ser-336 allows the kinase to switch to a Ca(2+)/calmodulin-independent state. Most likely the kinase is inactivated by the serine/ threonine protein phosphatase 2A. CaMK IV is a monomer that is located within the cytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei. In spermatids CaMK IV is associated with chromatin and the nuclear matrix. CaMK IV is also specifically expressed in epithelial ovarian cancer tissue.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
IF, 1:100-1:500
Validation Images & Assay Conditions
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Western Blot (WB) analysis using PDH-E1alpha Monoclonal antibody against A549, HeLa cell lysate.
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Immunofluorescence (IF) analysis of HeLa cells using PDH-E1alpha Monoclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PDHA1 (Source: Uniprot.org, NCBI)
Gene Name
PDHA1
Full Name
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
Weight
43.296kDa
Alternative Names
EC 1.2.4.1; PDHA; PDHE1-A type I; PHE1APDHCE1A; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial PDHA1 PDHA, PDHAD, PDHCE1A, PHE1A pyruvate dehydrogenase E1 subunit alpha 1 pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial|PDHE1-A type I|pyruvate dehydrogenase (lipoamide) alpha 1|pyruvate dehydrogenase E1 alpha 1 subunit|pyruvate dehydrogenase alpha 1|pyruvate dehydrogenase complex, E1-alpha polypeptide 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDHA1, check out the PDHA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDHA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDH-E1 alpha PDHA1 Monoclonal Antibody (A01906-2)
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No publications found for A01906-2
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