Product Info Summary
SKU: | A01906-3 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PDHA1 Antibody
View all Pyruvate Dehydrogenase E1-alpha subunit Antibodies
SKU/Catalog Number
A01906-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PDHA1 Antibody catalog # A01906-3. Tested in WB,ICC/IF,IHC,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDHA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01906-3)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PDHA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01906-3 is reactive to PDHA1 in Human, Mouse, Rat
Applications
A01906-3 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
43.296kDa
Background of Pyruvate Dehydrogenase E1-alpha subunit
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
Song B.J., Submitted (APR-1990).
Bentley D.R., Nature 434:325-337(2005).
Comb M.J., Cell 131:1190-1203(2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of PDHA1 using Anti-PDHA1 Antibody (A01906-3).
ICC staining PDHA1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-PDHA1 Antibody A01906-3
Western blot analysis of PDHA1 on different lysates using anti-PDHA1 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For PDHA1 (Source: Uniprot.org, NCBI)
Gene Name
PDHA1
Full Name
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
Weight
43.296kDa
Alternative Names
EC 1.2.4.1; PDHA; PDHE1-A type I; PHE1APDHCE1A; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial PDHA1 PDHA, PDHAD, PDHCE1A, PHE1A pyruvate dehydrogenase E1 subunit alpha 1 pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial|PDHE1-A type I|pyruvate dehydrogenase (lipoamide) alpha 1|pyruvate dehydrogenase E1 alpha 1 subunit|pyruvate dehydrogenase alpha 1|pyruvate dehydrogenase complex, E1-alpha polypeptide 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDHA1, check out the PDHA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDHA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDHA1 Antibody (A01906-3)
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No publications found for A01906-3
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Customer Reviews
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