Anti-Phospho-AMPK alpha1 (Ser496)PRKAA1 Antibody

Product Name

Anti-Phospho-AMPK alpha1 (Ser496)PRKAA1 Antibody

View all AMPK alpha 1 Antibodies

SKU/Catalog Number

P00994

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-AMPK alpha1 (Ser496)PRKAA1 Antibody (Catalog # P00994). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-AMPK alpha1 (Ser496)PRKAA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00994)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around phosphorylation site of serine 496 (S-G- S(P)-V-S) derived from Human AMPKα1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P00994 is reactive to PRKAA1 in Human

Applications

P00994 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

64.009kDa

Background of AMPK alpha 1

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 63kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

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Western blot analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPK alpha1 (Phospho-Ser496) antibody P00994.

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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPK alpha1 (Phospho-Ser496) antibody P00994 (left) or the same antibody preincubated with blocking peptide (right).

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Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPK alpha1 (Phospho-Ser496) antibody P00994.

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Immunofluorescence staining of methanol-fixed Hela cells using AMPK alpha1(Phospho-Ser496) antibody P00994.

Gene/Protein Information For PRKAA1 (Source: Uniprot.org, NCBI)

Alternative Names

AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK alpha 1; AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain; AMPK subunit alpha-1; AMPK; AMPK1; AMPKa1; EC 2.7.11; EC 2.7.11.1; MGC33776; MGC57364; PRKAA1; protein kinase, AMP-activated, alpha 1 catalytic subunit PRKAA1 AMPK, AMPKa1 protein kinase AMP-activated catalytic subunit alpha 1 5-AMP-activated protein kinase catalytic subunit alpha-1|5-AMP-activated protein kinase, catalytic alpha-1 chain|ACACA kinase|AMP -activate kinase alpha 1 subunit|AMP-activated protein kinase, catalytic, alpha-1|AMPK alpha 1|AMPK subunit alpha-1|HMGCR kinase|acetyl-CoA carboxylase kinase|hydroxymethylglutaryl-CoA reductase kinase|protein kinase, AMP-activated, alpha 1 catalytic subunit|tau-protein kinase PRKAA1

For more info on PRKAA1, check out the PRKAA1 Infographic

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In this infographic, you will see the following information for PRKAA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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