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Product Info Summary
| SKU: | A00031S339-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Fusin (S339) CXCR4 Antibody
SKU/Catalog Number
A00031S339-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Fusin (S339) CXCR4 Antibody catalog # A00031S339-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Fusin (S339) CXCR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00031S339-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CXCR4 around the phosphorylation site of Ser339. AA range:303-352
Reactive Species
A00031S339-1 is reactive to CXCR4 in Human, Monkey, Mouse, Rat
Calculated molecular weight
39.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00031S339-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of HuvEc etoposide 25uM 24H cells using Phospho-Fusin (S339) Polyclonal Antibody
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Western blot analysis of lysates from HUVEC cells treated with etoposide 25uM 24H, using CXCR4 (Phospho-Ser339) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using CXCR4 (Phospho-Ser339) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunofluorescence analysis of Hela cell. 1, Fusin (phospho Ser339) Polyclonal Antibody (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CXCR4 (Phospho-Ser339) Antibody
Specific Publications For Anti-Phospho-Fusin (S339) CXCR4 Antibody (A00031S339-1)
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