Product Info Summary
SKU: | A00807Y497 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Phospho-IL-4 alpha (Y497) IL4R Antibody
View all IL-4R alpha Antibodies
SKU/Catalog Number
A00807Y497
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-IL-4 alpha (Y497) IL4R Antibody catalog # A00807Y497. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-IL-4 alpha (Y497) IL4R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00807Y497)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human IL-4Ralpha around the phosphorylation site of Y497.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00807Y497 is reactive to IL4R in Human, Mouse
Applications
A00807Y497 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
89.658kDa
Background of IL-4R alpha
Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.
Arja Lamberg, J. Biol. Chem., May 1996; 271: 11988.
GW Conrad, J. Cell Biol., Mar 1980; 84: 501.
DR Keene, J. Cell Biol., Nov 1987; 105: 2393.
HM Wang, J. Histochem. Cytochem., Nov 1980; 28: 1215.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of 293 cells using Phospho-IL-4R Alpha (Y497) Polyclonal Antibody
Click image to see more details
Western blot analysis of lysates from 293 cells, using IL-4R/CD124 (Phospho-Tyr497) Antibody. The lane on the right is blocked with the phospho peptide.
Click image to see more details
Immunofluorescence analysis of HeLa cells, using IL-4R/CD124 (Phospho-Tyr497) Antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For IL4R (Source: Uniprot.org, NCBI)
Gene Name
IL4R
Full Name
Interleukin-4 receptor subunit alpha
Weight
89.658kDa
Superfamily
type I cytokine receptor family
Alternative Names
CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha IL4R CD124, IL-4RAA, IL4R interleukin 4 receptor interleukin-4 receptor subunit alpha|IL-4 receptor subunit alpha|IL4R nirs variant 1|interleukin 13 receptor|interleukin-4 receptor alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL4R, check out the IL4R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL4R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-IL-4 alpha (Y497) IL4R Antibody (A00807Y497)
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No publications found for A00807Y497
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