Anti-Phospho-LEF-1 (S42) Antibody

Product Name

Anti-Phospho-LEF-1 (S42) Antibody

View all Lef1 Antibodies

SKU/Catalog Number

A00605S42-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho-LEF-1 (S42) Antibody catalog # A00605S42-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-LEF-1 (S42) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00605S42-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human LEF-1 around the phosphorylation site of S42.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00605S42-1 is reactive to Lef1 in Human, Mouse, Rat

Applications

A00605S42-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

44.059kDa

Background of Lef1

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and Anti diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements. Anti-Ppar Antibody is useful for research interested in transcription and metabolic pathways.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ELISA 1:5000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-Phospho-LEF-1 (S42) Antibody A00605S42-1

Western Blot (WB) analysis of 293 cells using Phospho-LEF-1 (S42) polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For Lef1 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp586H0919; FLJ46390; LEF1; LEF-1; lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF10; TCF1ALPHA; TCF1-alpha; TCF7L3 Lef1|3000002B05, AI451430, Lef-, Lef-1|lymphoid enhancer binding factor 1|lymphoid enhancer-binding factor 1

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