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Product Info Summary
| SKU: | P01033 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody
SKU/Catalog Number
P01033
BM4727 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody catalog # P01033. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01033)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HEG-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Paxillin
Reactive Species
P01033 is reactive to PXN in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
64.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01033 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: HeLa cell, HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Paxillin (Y118) expression in (1) HeLa cell lysate; (2) HeLa cell treated with pervanadate.
Specific Publications For Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody (P01033)
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody
Question
We need using your anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody for cell adhesion studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
We appreciate your inquiry. This P01033 anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-31
Question
My colleagues were happy with the WB result of your anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody. However we have been able to see positive staining in brain testis cytoskeleton using this antibody. Is that expected? Could you tell me where is PXN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Based on literature, brain testis does express PXN. Generally PXN expresses in cytoplasm, cytoskeleton. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039
Boster Scientific Support
Answered: 2019-07-25
Question
We have been able to see staining in human brain testis. What should we do? Is anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody supposed to stain brain testis positively?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
From what I have seen in literature brain testis does express PXN. From what I have seen in Uniprot.org, PXN is expressed in fundus of stomach, placenta, fetal kidney, brain testis, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039
Boster Scientific Support
Answered: 2019-05-17
Question
We are currently using anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody P01033 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
S. Evans
Verified customer
Asked: 2017-03-28
Answer
The anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody (P01033) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-28
