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Product Info Summary
| SKU: | P01033 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody
SKU/Catalog Number
P01033
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody catalog # P01033. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01033)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HEG-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Paxillin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01033 is reactive to PXN in Human, Mouse, Rat
Applications
P01033 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
80 kDa
Calculated molecular weight
64.505kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Paxillin (Y118) expression in (1) HeLa cell lysate; (2) HeLa cell treated with pervanadate.
Protein Target Info & Infographic
Gene/Protein Information For PXN (Source: Uniprot.org, NCBI)
Gene Name
PXN
Full Name
Paxillin
Weight
64.505kDa
Superfamily
paxillin family
Alternative Names
FLJ16691; Paxillin; PXN PXN paxillin paxillin|testicular tissue protein Li 134
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PXN, check out the PXN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PXN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody (P01033)
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No publications found for P01033
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-Paxillin (Y118) PXN Rabbit Monoclonal Antibody
Question
We need using your anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody for cell adhesion studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
We appreciate your inquiry. This P01033 anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-31
Question
My colleagues were happy with the WB result of your anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody. However we have been able to see positive staining in brain testis cytoskeleton using this antibody. Is that expected? Could you tell me where is PXN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Based on literature, brain testis does express PXN. Generally PXN expresses in cytoplasm, cytoskeleton. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039
Boster Scientific Support
Answered: 2019-07-25
Question
We have been able to see staining in human brain testis. What should we do? Is anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody supposed to stain brain testis positively?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
From what I have seen in literature brain testis does express PXN. From what I have seen in Uniprot.org, PXN is expressed in fundus of stomach, placenta, fetal kidney, brain testis, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039
Boster Scientific Support
Answered: 2019-05-17
Question
We are currently using anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody P01033 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
S. Evans
Verified customer
Asked: 2017-03-28
Answer
The anti-Phospho-Paxillin (Y118) Rabbit Monoclonal antibody (P01033) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-28
