Anti-Phospho-PERK (T981) Rabbit Polyclonal Antibody

Product Name

Anti-Phospho-PERK (T981) Rabbit Polyclonal Antibody

View all PERK Antibodies

SKU/Catalog Number

P01992

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Phospho-PERK (T981) Rabbit Polyclonal Antibody catalog # P01992. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-PERK (T981) Rabbit Polyclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01992)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

608

Isotype

IgG

Immunogen

Synthesized peptide derived from human PERK around the phosphorylation site of T981. At AA range: 920-1000

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P01992 is reactive to EIF2AK3 in Human, Mouse, Rat

Applications

P01992 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

125.216kDa

Background of PERK

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr-1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-2000
IHC-P 1:100-1:300
IF 1:50-200
ELISA 1:40000

Validation Images & Assay Conditions

Click image to see more details

Western Blot (WB) analysis of specific cells using antibody diluted at 1:1000.

Click image to see more details

Western blot (WB) analysis of PERK (phospho Thr981) polyclonal antibody.

Click image to see more details

Immunohistochemical analysis of human-uterus tissue. Anti-Phospho-PERK (T981) at 1:200 (4°C, overnight). Antigen retrieval -Sodium Citrate pH6 (>98°C, 20min). Secondary -1:200 (room temp, 30min). Negative control -Secondary only

Click image to see more details

Immunohistochemical analysis of mouse kidney tissue. Anti-Phospho-PERK (T981) at 1:200 (4°C, overnight). Antigen retrieval -Sodium Citrate pH6 (>98°C, 20min). Secondary -1:200 (room temp, 30min). Negative control -Secondary only

Click image to see more details

Immunohistochemical analysis of mouse lung tissue. Anti-Phospho-PERK (T981) at 1:200 (4°C, overnight). Antigen retrieval -Sodium Citrate pH6 (>98°C, 20min). Secondary -1:200 (room temp, 30min). Negative control -Secondary only

Click image to see more details

Immunohistochemical analysis of rat kidney tissue. Anti-Phospho-PERK (T981) at 1:200 (4°C, overnight). Antigen retrieval -Sodium Citrate pH6 (>98°C, 20min). Secondary -1:200 (room temp, 30min). Negative control -Secondary only

Click image to see more details

Immunohistochemical analysis of rat lung tissue. Anti-Phospho-PERK (T981) at 1:200 (4°C, overnight). Antigen retrieval -Sodium Citrate pH6 (>98°C, 20min). Secondary -1:200 (room temp, 30min). Negative control -Secondary only

Click image to see more details

Immunohistochemical analysis of rat spleen tissue. Anti-Phospho-PERK (T981) at 1:200 (4°C, overnight). Antigen retrieval -Sodium Citrate pH6 (>98°C, 20min). Secondary -1:200 (room temp, 30min). Negative control -Secondary only

Click image to see more details

Immunohistochemical analysis of paraffin-embedded Human prostate cancer. Antibody was diluted at 1:100 (4°, overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antibody retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.

Gene/Protein Information For EIF2AK3 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp781H1925; EC 2.7.11.1; EIF2AK3; eukaryotic translation initiation factor 2 alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; hsPEK; PEK; PEKPERKPancreatic eIF2-alpha kinase; PERK; PRKR-like endoplasmic reticulum kinase; WRS EIF2AK3 PEK, PERK, WRS eukaryotic translation initiation factor 2 alpha kinase 3 eukaryotic translation initiation factor 2-alpha kinase 3|PRKR-like endoplasmic reticulum kinase|pancreatic EIF2-alpha kinase|truncated eukaryotic translation initiation factor 2 alpha kinase 3

For more info on EIF2AK3, check out the EIF2AK3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EIF2AK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the EIF2AK3 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis