Anti-PI 3 kinase p110 alpha Monoclonal Antibody

Product Name

Anti-PI 3 kinase p110 alpha Monoclonal Antibody

View all PIK3CA Antibodies

SKU/Catalog Number

M00029

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PI 3 kinase p110 alpha Monoclonal Antibody catalog # M00029. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PI 3 kinase p110 alpha Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00029)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BEB-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PI 3 Kinase catalytic subunit alpha

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00029 is reactive to PIK3CA in Human, Mouse, Rat

Applications

M00029 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

131 kDa

Calculated molecular weight

124.284kDa

Background of PIK3CA

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
ICC/IF 1:50-1:200
IP 1:20

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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Western blot analysis of PI 3 kinase p110 alpha expression in Jurkat cell lysate.Western blot analysis of PI 3 kinase p110 alpha expression in Jurkat cell lysate.

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Gene/Protein Information For PIK3CA (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.1; EC 2.7.1.153; MGC142161; MGC142163; p110-alpha; Phosphatidylinositol 3-Kinase catalytic subunit alpha; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform; phosphoinositide-3-kinase, catalytic, alpha polypeptide; PI3K; PI3K-alpha; PI3-kinase p110 subunit alpha; PI3-kinase subunit alp PIK3CA CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform|PI3-kinase p110 subunit alpha|phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha|phosphatidylinositol 3-kinase, catalytic, alpha polypeptide|phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha|phosphoinositide 3-kinase alpha|phosphoinositide-3-kinase, catalytic, alpha polypeptide|ptdIns-3-kinase subunit p110-alpha|serine/threonine protein kinase PIK3CA

For more info on PIK3CA, check out the PIK3CA Infographic

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In this infographic, you will see the following information for PIK3CA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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