Product Info Summary
SKU: | A01940 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-PKC beta 1/PRKCB Antibody Picoband™
SKU/Catalog Number
A01940
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PKC beta 1/PRKCB Antibody Picoband™ catalog # A01940. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC beta 1/PRKCB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01940)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PKC beta 1/PRKCB, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01940 is reactive to PRKCB in Human, Mouse, Rat
Applications
A01940 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
77 kDa
Calculated molecular weight
76.869kDa
Background of PRKCB
Protein kinase C beta type is an enzyme that in humans is encoded by the PRKCB gene. It is a member of the protein kinase C (PKC) gene family. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. It has been found that PRKCB activated by oxidative conditions in the cell, induces phosphorylation of p66 (SHC) and triggers mitochondrial accumulation of the protein after it is recognized by the prolyl isomerase PIN1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysate,
Lane 2: human THP-1 whole cell lysate,
Lane 3: human HEK293 whole cell lysate,
Lane 4: human Jurkat whole cell lysate,
Lane 5: human Raji whole cell lysate,
Lane 6: human HL-60 whole cell lysate,
Lane 7: human A549 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC beta 1 antigen affinity purified polyclonal antibody (Catalog # A01940) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC beta 1 at approximately 77KD. The expected band size for PKC beta 1 is at 77KD.
Click image to see more details
Figure 2. Western blot analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysate,
Lane 2: rat spleen tissue lysate,
Lane 3: rat brain tissue lysate,
Lane 4: rat C6 whole cell lysate,
Lane 5: mouse thymus tissue lysate,
Lane 6: mouse spleen tissue lysate,
Lane 7: mouse brain tissue lysate,
Lane 8: mouse Neuro-2a whole cell lysate,
Lane 9: mouse NIH3T3 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC beta 1 antigen affinity purified polyclonal antibody (Catalog # A01940) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC beta 1 at approximately 77KD. The expected band size for PKC beta 1 is at 77KD.
Click image to see more details
Figure 3. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC beta 1 Antibody (A01940) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC beta 1 Antibody (A01940) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC beta 1 Antibody (A01940) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of human prostatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC beta 1 Antibody (A01940) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC beta 1 Antibody (A01940) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PRKCB (Source: Uniprot.org, NCBI)
Gene Name
PRKCB
Full Name
Protein kinase C beta type
Weight
76.869kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.13; MGC41878; PKC-B; PKC-beta; PKCBPRKCB2; PRKCB1protein kinase C beta 1; protein kinase C beta type; protein kinase C, beta 1 polypeptide; protein kinase C, beta 1; protein kinase C, beta PRKCB PKC-beta, PKCB, PKCI(2), PKCbeta1, PRKCB2, PRKCB protein kinase C beta protein kinase C beta type|PKC-B|protein kinase C, beta 1 polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCB, check out the PRKCB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKC beta 1/PRKCB Antibody Picoband™ (A01940)
Hello CJ!
A01940 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors
5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors
Different localization and expression of protein kinase C-beta in kidney cortex of diabetic nephropathy mice and its role in telmisartan treatment
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