Click image to see more details
Product Info Summary
| SKU: | M00961-Biotin |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cystatin C/CST3 Picoband™ Biotinylated Antibody (monoclonal, 8D1)
View all Cystatin C Antibodies
SKU/Catalog Number
M00961-Biotin
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cystatin C/CST3 Picoband™ Biotinylated Antibody (monoclonal, 8D1) catalog # M00961-Biotin. Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cystatin C/CST3 Picoband™ Biotinylated Antibody (monoclonal, 8D1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00961-Biotin)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
8D1
Isotype
Mouse IgG1
Immunogen
E. coli-derived human Cystatin C recombinant protein (Position: K31-A146).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00961-Biotin is reactive to CST3 in Human
Applications
M00961-Biotin is guaranteed for ELISA Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
15.799kDa
Background of Cystatin C
Cystatin C or cystatin 3, a protein encoded by the CST3 gene, is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and body fluids. It is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Cystatin C belongs to the type 2 cystatin gene family.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (Det), 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CST3 (Source: Uniprot.org, NCBI)
Gene Name
CST3
Full Name
Cystatin-C
Weight
15.799kDa
Superfamily
cystatin family
Alternative Names
ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin CST3 ARMD11, HEL-S-2 cystatin C cystatin-C|bA218C14.4 (cystatin C)|cystatin 3|epididymis secretory protein Li 2|gamma-trace|neuroendocrine basic polypeptide|post-gamma-globulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CST3, check out the CST3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CST3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cystatin C/CST3 Picoband™ Biotinylated Antibody (monoclonal, 8D1) (M00961-Biotin)
Hello CJ!
No publications found for M00961-Biotin
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cystatin C/CST3 Picoband™ Biotinylated Antibody (monoclonal, 8D1)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Cystatin C/CST3 Picoband™ Biotinylated Antibody (monoclonal, 8D1)
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Cystatin C/CST3 Picoband™ Biotinylated Antibody (monoclonal, 8D1)
Question
I was wanting to use your anti-Cystatin C/CST3 Biotinylated antibody (monoclonal, 8D1) for ELISA for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
It shows on the product datasheet, M00961-Biotin anti-Cystatin C/CST3 Biotinylated antibody (monoclonal, 8D1) has been validated for ELISA on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-12
Question
We are currently using anti-Cystatin C/CST3 Biotinylated antibody (monoclonal, 8D1) M00961-Biotin for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
The anti-Cystatin C/CST3 Biotinylated antibody (monoclonal, 8D1) (M00961-Biotin) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-01
Question
Does anti-Cystatin C/CST3 Biotinylated antibody (monoclonal, 8D1) M00961-Biotin work on bovine ELISA with cerebrospinal fluid?
L. Li
Verified customer
Asked: 2019-04-19
Answer
Our lab technicians have not tested anti-Cystatin C/CST3 Biotinylated antibody (monoclonal, 8D1) M00961-Biotin on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Cystatin C/CST3 Biotinylated antibody (monoclonal, 8D1) M00961-Biotin to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cerebrospinal fluid in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-19
