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Product Info Summary
| SKU: | M01940 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody
SKU/Catalog Number
M01940
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody catalog # M01940. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01940)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DOC-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PKC beta 2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01940 is reactive to PRKCB in Human, Mouse, Rat
Applications
M01940 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
28 kDa
Calculated molecular weight
76.869kDa
Background of PRKCB
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of PKC beta 2 expression in K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PRKCB (Source: Uniprot.org, NCBI)
Gene Name
PRKCB
Full Name
Protein kinase C beta type
Weight
76.869kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.13; MGC41878; PKC-B; PKC-beta; PKCBPRKCB2; PRKCB1protein kinase C beta 1; protein kinase C beta type; protein kinase C, beta 1 polypeptide; protein kinase C, beta 1; protein kinase C, beta PRKCB PKC-beta, PKCB, PKCI(2), PKCbeta1, PRKCB2, PRKCB protein kinase C beta protein kinase C beta type|PKC-B|protein kinase C, beta 1 polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCB, check out the PRKCB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody (M01940)
Hello CJ!
M01940 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Different localization and expression of protein kinase C-beta in kidney cortex of diabetic nephropathy mice and its role in telmisartan treatment
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-PKC beta 2 Rabbit Monoclonal antibody for IP for human platelet on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human platelet identification?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
You can see on the product datasheet, M01940 anti-PKC beta 2 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
Would anti-PKC beta 2 Rabbit Monoclonal antibody M01940 work on zebrafish WB with putamen?
T. Williams
Verified customer
Asked: 2019-06-26
Answer
Our lab technicians have not validated anti-PKC beta 2 Rabbit Monoclonal antibody M01940 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PKC beta 2 Rabbit Monoclonal antibody M01940 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish putamen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-26
Question
Is there a BSA free version of anti-PKC beta 2 Rabbit Monoclonal antibody M01940 available?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PKC beta 2 Rabbit Monoclonal antibody M01940 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-07
Question
Would M01940 anti-PKC beta 2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Brown
Verified customer
Asked: 2013-10-31
Answer
As indicated on the product datasheet, M01940 anti-PKC beta 2 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-10-31
