Anti-POLR2A (Phospho-S2) Antibody

Product Name

Anti-POLR2A (Phospho-S2) Antibody

View all RNA Polymerase II/POLR2A Antibodies

SKU/Catalog Number

A01029S2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-POLR2A (Phospho-S2) Antibody catalog # A01029S2. Tested in WB,IP,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-POLR2A (Phospho-S2) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01029S2)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human IRAK-1 around the phosphorylation site of T209.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01029S2 is reactive to POLR2A in Human, Mouse, Rat

Applications

A01029S2 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

217.176kDa

Background of RNA Polymerase II/POLR2A

4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Gingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of POLR2A using Anti-POLR2A (Phospho-S2) Antibody (A01029S2).

ICC staining Phospho-POLR2A (S2) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-POLR2A (Phospho-S2) Antibody A01029S2

Western blot analysis of Phospho-POLR2A (S2) on differnt cells lysates using anti-Phospho-POLR2A (S2) antibody at 1/500 dilution. Positive control：
Line 1: Hela
Line 2: MCF-7
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For POLR2A (Source: Uniprot.org, NCBI)

Alternative Names

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.48; EC 2.7.7.6; hRPB220; hsRPB1; MGC75453; POLR2; POLR2A; POLRA; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; RNA Pol 2; RNA Polymerase 2; RNA polymerase II subunit B1; RNA Polymerase II; RNA-directed RNA polymerase II subunit RPB1; RNAPII; RPB1; RPBh1; RpIILS; RPO2; RPOL2 POLR2A NEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1 RNA polymerase II subunit A DNA-directed RNA polymerase II subunit RPB1|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|DNA-directed RNA polymerase II subunit A|DNA-directed RNA polymerase III largest subunit|RNA polymerase II subunit B1|RNA-directed RNA polymerase II subunit RPB1|polymerase (RNA) II (DNA directed) polypeptide A, 220kDa|polymerase (RNA) II subunit A

For more info on POLR2A, check out the POLR2A Infographic

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In this infographic, you will see the following information for POLR2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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