Product Info Summary
SKU: | A03026-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | WB |
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Product info
Product Name
Anti-PP2B-A alpha PPP3CA Monoclonal Antibody
View all Calcineurin A Antibodies
SKU/Catalog Number
A03026-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PP2B-A alpha PPP3CA Monoclonal Antibody catalog # A03026-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PP2B-A alpha PPP3CA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03026-1)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 190-236.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03026-1 is reactive to PPP3CA in Human, Mouse, Rat
Applications
A03026-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
58.688kDa
Background of Calcineurin A
Anti-Cul2 antibody can be used in western blotting, ELISA and IP. Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 2 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul2 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul2 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul2 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul2 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis using PP2B-Aalpha Monoclonal antibody against A431, HeLa cell lysate .
Protein Target Info & Infographic
Gene/Protein Information For PPP3CA (Source: Uniprot.org, NCBI)
Gene Name
PPP3CA
Full Name
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Weight
58.688kDa
Superfamily
PPP phosphatase family
Alternative Names
calcineurin A alpha; Calcineurin A; Calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA; CALNAcatalytic subunit, alpha isoform; CAM-PRP catalytic subunit; CNA; CNA1; CNA1CALNA1; EC 3.1.3.16; PP2B; PP3CA; PPP2B; PPP2BCCN1; PPP3CA; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha); protein phosphatase 3, catalytic subunit, alpha isozyme; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform PPP3CA ACCIID, CALN, CALNA, CALNA1, CCN1, CNA1, DEE91, IECEE, IECEE1, PPP2B protein phosphatase 3 catalytic subunit alpha serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform|CAM-PRP catalytic subunit|calcineurin A alpha|protein phosphatase 3 catalytic subunit alpha isozyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP3CA, check out the PPP3CA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP3CA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PP2B-A alpha PPP3CA Monoclonal Antibody (A03026-1)
Hello CJ!
A03026-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Buckwheat Rutin Inhibits AngII-induced Cardiomyocyte Hypertrophy via Blockade of CaN-dependent Signal Pathway
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-PP2B-A alpha PPP3CA Monoclonal Antibody
Question
We were happy with the WB result of your anti-PP2B-A alpha Monoclonal antibody . However we have observed positive staining in pancreas cytoplasm using this antibody. Is that expected? Could you tell me where is PPP3CA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
According to literature, pancreas does express PPP3CA. Generally PPP3CA expresses in cytoplasm. Regarding which tissues have PPP3CA expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 8392375, 14702039
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-27
Question
My lab would like using your anti-PP2B-A alpha Monoclonal antibody for skeletal muscle tissue regeneration studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
C. Carter
Verified customer
Asked: 2019-11-08
Answer
We appreciate your inquiry. This A03026-1 anti-PP2B-A alpha Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-08
Question
Can you help my question with product A03026-1, anti-PP2B-A alpha Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03026-1 anti-PP2B-A alpha Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-29
Question
I was wanting to use your anti-PP2B-A alpha Monoclonal antibody for WB for human pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas identification?
D. Walker
Verified customer
Asked: 2019-09-11
Answer
You can see on the product datasheet, A03026-1 anti-PP2B-A alpha Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-11
Question
I am interested in to test anti-PP2B-A alpha Monoclonal antibody A03026-1 on human pancreas for research purposes, then I may be interested in using anti-PP2B-A alpha Monoclonal antibody A03026-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Jones
Verified customer
Asked: 2019-04-05
Answer
The products we sell, including anti-PP2B-A alpha Monoclonal antibody A03026-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-05
Question
I see that the anti-PP2B-A alpha Monoclonal antibody A03026-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-05
Question
Does anti-PP2B-A alpha Monoclonal antibody A03026-1 work for WB with pancreas?
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
According to the expression profile of pancreas, PPP3CA is highly expressed in pancreas. So, it is likely that anti-PP2B-A alpha Monoclonal antibody A03026-1 will work for WB with pancreas.
Boster Scientific Support
Answered: 2018-07-05
Question
Is there a BSA free version of anti-PP2B-A alpha Monoclonal antibody A03026-1 available?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PP2B-A alpha Monoclonal antibody A03026-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-01
Question
Please see the WB image, lot number and protocol we used for pancreas using anti-PP2B-A alpha Monoclonal antibody A03026-1. Please let me know if you require anything else.
S. Collins
Verified customer
Asked: 2016-08-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-PP2B-A alpha Monoclonal antibody A03026-1. Let me know if you need anything else.
L. Moore
Verified customer
Asked: 2016-02-08
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-02-08
Question
We are currently using anti-PP2B-A alpha Monoclonal antibody A03026-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
W. Parker
Verified customer
Asked: 2014-03-18
Answer
The anti-PP2B-A alpha Monoclonal antibody (A03026-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-18
Question
Is a blocking peptide available for product anti-PP2B-A alpha Monoclonal antibody (A03026-1)?
E. Li
Verified customer
Asked: 2013-07-02
Answer
We do provide the blocking peptide for product anti-PP2B-A alpha Monoclonal antibody (A03026-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-07-02
Question
We have been able to see staining in mouse brain. What should we do? Is anti-PP2B-A alpha Monoclonal antibody supposed to stain brain positively?
A. Krishna
Verified customer
Asked: 2013-04-05
Answer
Based on literature brain does express PPP3CA. Based on Uniprot.org, PPP3CA is expressed in caudate nucleus, brain, amygdala, pancreas, erythroleukemia, among other tissues. Regarding which tissues have PPP3CA expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 8392375, 14702039
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-04-05
Question
Does A03026-1 anti-PP2B-A alpha Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Lewis
Verified customer
Asked: 2013-01-24
Answer
You can see on the product datasheet, A03026-1 anti-PP2B-A alpha Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-01-24