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Product Info Summary
| SKU: | A00412-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PRDM1 BLIMP1 Antibody
View all BLIMP1/PRDM1 Antibodies
SKU/Catalog Number
A00412-2
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-PRDM1 BLIMP1 Antibody (Catalog # A00412-2). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store Blimp1 antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-PRDM1 BLIMP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00412-2)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-Blimp1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of human, mouse, and rat PRDM1/BLIMP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00412-2 is reactive to PRDM1 in Human
Applications
A00412-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
91.771kDa
Background of BLIMP1/PRDM1
Anti-Blimp1 is ideal for Immunology, Epigenetic and Nuclear Signaling research. PRDM1/Blimp1 (PR domain zinc finger protein 1, PR domain-containing protein 1, Beta-interferon gene positive regulatory domain I-binding factor, BLIMP-1, Positive regulatory domain I-binding factor 1, PRDI-binding factor 1, or PRDI-BF1, or Beta-lymphocyte-induced maturation protein 1) is one of several transcription factors which drives the maturation of B-lymphocytes into Ig secreting cells. In the nucleus, PRDM1/BLIMP1 functions as a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Transcription of the PRDM1 gene is increased upon virus induction. PRDM1/Blimp1 also interacts with PRMT5. Two alternatively spliced transcript variants that encode different isoforms have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000 - 1:40,000
IHC: 5µg/mL
IP: 5-10 µg/mL
WB: 1:1,000
Validation Images & Assay Conditions
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Figure 1. IHC analysis of PRDM1 using anti-PRDM1 antibody (A00412-2).
PRDM1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PRDM1 Antibody (A00412-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of PRDM1 using anti-PRDM1 antibody (A00412-2).
PRDM1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PRDM1 Antibody (A00412-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Western blot analysis of PRDM1 using anti-PRDM1 antibody (A00412-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM1 antigen affinity purified polyclonal antibody (Catalog # A00412-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PRDM1.
Protein Target Info & Infographic
Gene/Protein Information For PRDM1 (Source: Uniprot.org, NCBI)
Gene Name
PRDM1
Full Name
PR domain zinc finger protein 1
Weight
91.771kDa
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP1; BLIMP-1; BLIMP1MGC118925; blmp-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI-BF1MGC118924; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1; PR-domain zinc finger protein 1; ZNFPR1A1 PRDM1 BLIMP1, PRDI-BF1 PR/SET domain 1 PR domain zinc finger protein 1|B-lymphocyte-induced maturation protein 1|BLIMP-1|PR domain 1|PR domain containing 1, with ZNF domain|PRDI-binding factor-1|beta-interferon gene positive-regulatory domain I binding factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRDM1, check out the PRDM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRDM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRDM1 BLIMP1 Antibody (A00412-2)
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