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Product Info Summary
| SKU: | M00334 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00334
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody catalog # M00334. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human pro Caspase 3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00334 is reactive to CASP3 in Human, Mouse
Applications
M00334 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
47 kDa
Calculated molecular weight
31.608kDa
Background of Caspase-3
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of pro Caspase 3 in HeLa cell lysate.
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Immunofluorescent analysis of A673 cells, using pro Caspase 3 Antibody.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For CASP3 (Source: Uniprot.org, NCBI)
Gene Name
CASP3
Full Name
Caspase-3
Weight
31.608kDa
Superfamily
peptidase C14A family
Alternative Names
Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA CASP3 CPP32, CPP32B, SCA-1 caspase 3 caspase-3|CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine peptidase|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|protein Yama
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP3, check out the CASP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody (M00334)
Hello CJ!
M00334 has been cited in 32 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu DX,Li PP,Guo JP,Li LL,Guo B,Jiao HB,Wu JH,Chen JM.Exosomes derived from HBV-associated liver cancer promote chemoresistance by upregulating chaperone-mediated autophagy.Oncol Lett.2019 Jan;17(1):323-331.doi:10.3892/ol.2018.9584.Epub 2018 Oct 16.PMID:30655770; PMCID: PMC6313222.
Species: Human
M00334 usage in article: APP:WB, SAMPLE:HEPG2 CELL, DILUTION:1:500
Jia-Hui Yan,Yi-Shun Ji,Man-Li Yang,Jun Fu,Hu Shan,Li−Li Wang,Lei Zhang,Jing-Yuan Xiao,Xiao-Ying Kong, and Jin-Sheng Shi ACS Applied Nano Materials 2020 3 (9), 8817-8828 DOI: 10.1021/acsanm.0c01607
Species: Cuttlefish,Human,Mouse
M00334 usage in article: APP:IHC, SAMPLE:HEART,LIVER,SPLEEN,LUNG AND KIDNEY, DILUTION:NA
Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse
M00334 usage in article: APP:WB, SAMPLE:SNU-16 CELL, DILUTION:NA
Sun K,Luo J,Jing X,Xiang W,Guo J,Yao X,Liang S,Guo F,Xu T.Hyperoside ameliorates the progression of osteoarthritis: An in vitro and in vivo study.Phytomedicine.2020 Oct 14;80:153387.doi:10.1016/j.phymed.2020. 153387.Epub ahead of print.PMID:33130473.
Species: Mouse
M00334 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:1:1000
Wei JL,Wu CJ,Chen JJ,Shang FT,Guo SG,Zhang XC,Liu H.LncRNA NEAT1 promotes the progression of sepsis-induced myocardial cell injury by sponging miR-144-3p.Eur Rev Med Pharmacol Sci.2020 Jan;24(2):851-861.doi:10.26355/eurrev_202001_20069.PMID:32016991.
Species: Mouse
M00334 usage in article: APP:WB, SAMPLE:HL-1 CELLS, DILUTION:1:1000
Zhu X,Yao Y,Yang J,Zhengxie J,Li X,Hu S,Zhang A,Dong J,Zhang C,Gan G.COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice. Int Immunopharmacol. 2020 Oct;87:106801.doi: 10.1016/j.inti
Species: Mouse
Zhou X,Li Y,Wu C,Yu W,Cheng F.Novel lncRNA XLOC_032768 protects against renal tubular epithelial cells apoptosis in renal ischemia-reperfusion injury by regulating FNDC3B/TGF-β1.Ren Fail.2020 Nov;42(1):994-1003.doi:10.1080/0 8860 22X.2020.1818579.PMID:329
Species: Mouse
M00334 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:NA
Wang P,Wang C,Liu C. Antitumor effects of dioscin in A431 cells via adjusting ATM/p53-mediated cell apoptosis, DNA damage and migration. Oncol Lett. 2021 Jan;21(1):59.doi:10.3892/ol.2020.12321.Epub 2020 Nov 19.PMID:33281970;PMCID:PMC7709553.
Species: Human
M00334 usage in article: APP:IF, SAMPLE:A431 CELLS, DILUTION:1:100
Chen L,Xu P,Xiao Q,Chen L,Li S,Jian JM,Zhong YB. Sunitinib malate inhibits intestinal tumor development in male ApcMin/+ mice by down-regulating inflammation-related factors with suppressing β-cateinin/c-Myc pathway and re-balancing Bcl-6 and Caspase-3. I
Species: Mouse
M00334 usage in article: APP:WB, SAMPLE:INTESTINES, DILUTION:1:600
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
Question
Will anti-pro Caspase 3 Rabbit Monoclonal antibody M00334 work for IP with jejunal mucosa?
G. Jha
Verified customer
Asked: 2020-04-29
Answer
According to the expression profile of jejunal mucosa, CASP3 is highly expressed in jejunal mucosa. So, it is likely that anti-pro Caspase 3 Rabbit Monoclonal antibody M00334 will work for IP with jejunal mucosa.
Boster Scientific Support
Answered: 2020-04-29
Question
We are currently using anti-pro Caspase 3 Rabbit Monoclonal antibody M00334 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
The anti-pro Caspase 3 Rabbit Monoclonal antibody (M00334) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-27
Question
Will M00334 anti-pro Caspase 3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
As indicated on the product datasheet, M00334 anti-pro Caspase 3 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-05
Question
I would like using your anti-pro Caspase 3 Rabbit Monoclonal antibody for response to lipopolysaccharide studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
Thank you for your inquiry. This M00334 anti-pro Caspase 3 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-12
Question
My question regards to test anti-pro Caspase 3 Rabbit Monoclonal antibody M00334 on human jejunal mucosa for research purposes, then I may be interested in using anti-pro Caspase 3 Rabbit Monoclonal antibody M00334 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The products we sell, including anti-pro Caspase 3 Rabbit Monoclonal antibody M00334, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-11
Question
Is a blocking peptide available for product anti-pro Caspase 3 Rabbit Monoclonal antibody (M00334)?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
We do provide the blocking peptide for product anti-pro Caspase 3 Rabbit Monoclonal antibody (M00334). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-22
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for jejunal mucosa using anti-pro Caspase 3 Rabbit Monoclonal antibody M00334. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-30
Question
We have observed staining in mouse tongue. Are there any suggestions? Is anti-pro Caspase 3 Rabbit Monoclonal antibody supposed to stain tongue positively?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
From literature tongue does express CASP3. From Uniprot.org, CASP3 is expressed in jejunal mucosa, t-cell, tongue, lymph, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-25
Question
My question regarding product M00334, anti-pro Caspase 3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00334 anti-pro Caspase 3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-23
Question
We bought anti-pro Caspase 3 Rabbit Monoclonal antibody for IP on lymph in a previous project. I am using human, and We intend to use the antibody for IF next. We need examining lymph as well as jejunal mucosa in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
I looked at the website and datasheets of our anti-pro Caspase 3 Rabbit Monoclonal antibody and it seems that M00334 has been validated on human in both IP and IF. Thus M00334 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-21
Question
Our lab were satisfied with the WB result of your anti-pro Caspase 3 Rabbit Monoclonal antibody. However we have observed positive staining in jejunal mucosa cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP3 supposed to be expressed?
J. Miller
Verified customer
Asked: 2019-05-01
Answer
From literature, jejunal mucosa does express CASP3. Generally CASP3 expresses in cytoplasm. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-05-01
Question
I see that the anti-pro Caspase 3 Rabbit Monoclonal antibody M00334 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-24
Question
Is this M00334 anti-pro Caspase 3 Rabbit Monoclonal antibody reactive to the isotypes of CASP3?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
The immunogen of M00334 anti-pro Caspase 3 Rabbit Monoclonal antibody is A synthesized peptide derived from human pro Caspase 3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-16
Question
Here is the WB image, lot number and protocol we used for jejunal mucosa using anti-pro Caspase 3 Rabbit Monoclonal antibody M00334. Please let me know if you require anything else.
B. Krishna
Verified customer
Asked: 2015-01-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-07
Question
Do you have a BSA free version of anti-pro Caspase 3 Rabbit Monoclonal antibody M00334 available?
N. Parker
Verified customer
Asked: 2014-06-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-pro Caspase 3 Rabbit Monoclonal antibody M00334 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-06-30
Question
I was wanting to use your anti-pro Caspase 3 Rabbit Monoclonal antibody for IP for human jejunal mucosa on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human jejunal mucosa identification?
J. Patel
Verified customer
Asked: 2014-06-26
Answer
You can see on the product datasheet, M00334 anti-pro Caspase 3 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human jejunal mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-26
