Product Info Summary
SKU: | M06262 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RAP80 Monoclonal Antibody
SKU/Catalog Number
M06262
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RAP80 Monoclonal Antibody catalog # M06262. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAP80 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06262)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADCH-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RAP80 Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs) .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06262 is reactive to Uimc1 in Human
Applications
M06262 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
70,90 kDa
Calculated molecular weight
81.478kDa
Background of RAP80
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of RAP80 expression in 293T cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For Uimc1 (Source: Uniprot.org, NCBI)
Gene Name
Uimc1
Full Name
BRCA1-A complex subunit RAP80
Weight
81.478kDa
Superfamily
RAP80 family
Alternative Names
BRCA1-A complex subunit RAP80; RAP80; RAP80X2HRIP110; receptor associated protein 80; Receptor-associated protein 80; retinoid x receptor interacting protein; Retinoid X receptor-interacting protein 110; RXRIP110; ubiquitin interaction motif containing 1; Ubiquitin interaction motif-containing protein 1; UIMC1; X2HRIP110 Uimc1|9430016E08Rik, D330018D10Rik, D630032M02Rik, RIP110, Rxri, Rxrip110|ubiquitin interaction motif containing 1|BRCA1-A complex subunit RAP80|receptor-associated protein 80|retinoid X receptor interacting protein 110|ubiquitin interaction motif-containing protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Uimc1, check out the Uimc1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Uimc1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAP80 Monoclonal Antibody (M06262)
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No publications found for M06262
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-RAP80 Monoclonal Antibody
Question
We are currently using anti-RAP80 Monoclonal antibody M06262 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
The anti-RAP80 Monoclonal antibody (M06262) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-18