Product Info Summary
SKU: | A07101-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Rho H (M134) Antibody
SKU/Catalog Number
A07101-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rho H (M134) Antibody catalog # A07101-1. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rho H (M134) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07101-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human TRIM3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07101-1 is reactive to RHOH in Human, Mouse, Rat
Applications
A07101-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
21.331kDa
Background of RHOH
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Shirane M, et al. (1999) J Biol Chem; 274(40): 28169-74
Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663.
Chen, Z.J. et al. (1996) Cell; 84, 853-862.
Brown, K. et al. (1995) Science; 267, 1485-1488.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of RHOH using Anti-Rho H (M134) Antibody (A07101-1).
Immunohistochemistry (IHC) analyzes of Rho H (M134) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50.showing cytoplasmic and cell membrane staining. Negative control (the right)Using PBS instead of primary antibodyClick image to see more details
Figure 1. Western blotting validation for Anti-Rho H (M134) Antibody A07101-1
Western blot (WB) analysis of Rho H (M134) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate(20ug)
Lane2:HepG2 whole cell lysate(20ug)
Lane3:Hela whole cell lysate(20ug)
Lane4:C6 whole cell lysate(40ug)
Lane5:CT26 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For RHOH (Source: Uniprot.org, NCBI)
Gene Name
RHOH
Full Name
Rho-related GTP-binding protein RhoH
Weight
21.331kDa
Superfamily
small GTPase superfamily
Alternative Names
ras homolog gene family, member H; translocation three four RHOH ARHH, TTF ras homolog family member H rho-related GTP-binding protein RhoH|GTP-binding protein TTF|TTF, translocation three four|ras homolog gene family, member H
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHOH, check out the RHOH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHOH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rho H (M134) Antibody (A07101-1)
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No publications found for A07101-1
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Customer Reviews
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