Anti-RIPK1 Antibody

Product Name

Anti-RIPK1 Antibody

View all RIPK1/RIP1 Antibodies

SKU/Catalog Number

A00141

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RIPK1 Antibody (Catalog # A00141). Tested in ELISA, IHC-P applications. This antibody reacts with Human.

Storage & Handling

RIPK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-RIPK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00141)

Host

Rabbit

Contents

RIPK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RIPK1 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human RIP1. The immunogen is located within amino acids 600 - 650 of RIPK1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

MMP9 antibody is human, mouse and rat reactive. At least three isoforms of MMP9 are known to exist; this antibody only recognizes the two longest isoforms.

Reactive Species

A00141 is reactive to RIPK1 in Human

Applications

A00141 is guaranteed for ELISA, IHC-P Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

75.931kDa

Background of RIPK1/RIP1

RIP1 (Receptor Interacting Protein), also known as RIPK1, is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIP1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIP1 is important for signallling to NF-kappa-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIP1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-kappa-B activation. RIP1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RIPK1 antibody can be used for detection of RIP1 by immunohistochemistry at 5 - 10 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Immunohistochemistry of RIP1 in human placenta tissue with RIP1 antibody at 10 μg/mL.

Gene/Protein Information For RIPK1 (Source: Uniprot.org, NCBI)

Alternative Names

Cell death protein RIP; EC 2.7.11.1; receptor (TNFRSF)-interacting serine-threonine kinase 1; receptor interacting protein; Receptor-interacting protein 1; receptor-interacting serine/threonine-protein kinase 1; RIP; Rip1 kinase; RIP1; RIP-1; RIPFLJ39204; RIPK1; Serine/threonine-protein kinase RIP RIPK1 AIEFL, IMD57, RIP, RIP-1, RIP1 receptor interacting serine/threonine kinase 1 receptor-interacting serine/threonine-protein kinase 1|cell death protein RIP|receptor (TNFRSF)-interacting serine-threonine kinase 1|receptor-interacting protein 1|receptor-interacting protein kinase 1|serine/threonine-protein kinase RIP

For more info on RIPK1, check out the RIPK1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RIPK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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