Anti-S100 Beta S100B Antibody

Product Name

Anti-S100 Beta S100B Antibody

View all S100B Antibodies

SKU/Catalog Number

A00979S100

Size

100ul

Form

Liquid

Description

Boster Bio Anti-S100 Beta S100B Antibody catalog # A00979S100. Tested in WB,IF,IP,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-S100 Beta S100B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00979S100)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human LRP6 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00979S100 is reactive to S100B in Human, Mouse, Rat

Applications

A00979S100 is guaranteed for IP, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

10.713kDa

Background of S100B

4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Gingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2000
IF: 1:50-1:200
IHC: 1:50-1:200
IP: 1:50-1:200

Validation Images & Assay Conditions

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ICC staining S100 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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Western blot analysis of S100 beta on different lysates using anti-S100 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver Lane 2: Mouse heart Lane 3: Hela

Gene/Protein Information For S100B (Source: Uniprot.org, NCBI)

Alternative Names

beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta S100B NEF, S100, S100-B, S100beta S100 calcium binding protein B protein S100-B|S-100 calcium-binding protein, beta chain|S-100 protein subunit beta|S100 calcium-binding protein, beta (neural)

For more info on S100B, check out the S100B Infographic

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In this infographic, you will see the following information for S100B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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