Anti-S100A11 Antibody Picoband™

Product Name

Anti-S100A11 Antibody Picoband™

View all S100A11 Antibodies

SKU/Catalog Number

PB9818

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-S100A11 Antibody Picoband™ catalog # PB9818. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-S100A11 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9818)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human S100A11 recombinant protein (Position: A2-T105). Human S100A11 shares 86.7% amino acid (aa) sequence identity with both mouse and rat S100A11.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9818 is reactive to S100A11 in Human

Applications

PB9818 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

15 kDa

Calculated molecular weight

11.74kDa

Background of S100A11

S100 calcium-binding protein A11 (S100A11) is a protein that in humans is encoded by the S100A11 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. And S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of S100A11 using anti-S100A11 antibody (PB9818).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A11 antigen affinity purified polyclonal antibody (Catalog # PB9818) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A11 at approximately 15 kDa. The expected band size for S100A11 is at 15 kDa.

Gene/Protein Information For S100A11 (Source: Uniprot.org, NCBI)

Alternative Names

Calgizzarin; Metastatic lymph node gene 70 protein; MLN 70; MLN70; protein S100-A11; Protein S100-C; S100 calcium binding protein A11; S100 calcium-binding protein A11 (calgizzarin); S100 calcium-binding protein A11; S100A11; S100C; S100CS100 calcium binding protein A11 (calgizzarin) S100A11 HEL-S-43, MLN70, S100C S100 calcium binding protein A11 protein S100-A11|MLN 70|calgizzarin|epididymis secretory protein Li 43|metastatic lymph node gene 70 protein|protein S100-C

For more info on S100A11, check out the S100A11 Infographic

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In this infographic, you will see the following information for S100A11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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