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Product Info Summary
| SKU: | A02558 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SESTRIN2 SESN2 Antibody
SKU/Catalog Number
A02558
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SESTRIN2 SESN2 Antibody (Catalog # A02558). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SESTRIN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-SESTRIN2 SESN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02558)
Host
Rabbit
Contents
SESTRIN2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
SESTRIN2 antibody was raised against a 19 amino acid peptide near the center of human SESTRIN2. The immunogen is located within amino acids 220 - 270 of SESTRIN2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SESTRIN2 antibody is human, mouse and rat reactive. At least two isoforms of SESTRIN2 are known to exist; this antibody will detect both isoforms. SESTRIN2 antibody is predicted to not cross-react with SESTRIN1.
Reactive Species
A02558 is reactive to SESN2 in Human, Mouse, Rat
Applications
A02558 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
54.494kDa
Background of SESN2
SESTRIN2, also known as Hi95, is a member of the sestrin family of PA26-related proteins and is induced following DNA damage or oxidative stress (1). SESTRIN2 and the related protein SESTRIN1 interact with the NRF2 suppressor KEAP1, the autophagy substrate p62 and the ubiquitin ligase RBX1 to protect cells against oxidative stress by activating NRF2 and promoting the p62-dependent autophagic degradation of KEAP1 (2). SESTRIN2 also negatively regulates TOR signaling by activating AMP-activated protein kinase (AMPK) and TSC2 phosphorylation (3), thereby protecting cells against energetic stress-induced death (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SESTRIN2 antibody can be used for detection of SESTRIN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SESTRIN2 in mouse kidney tissue lysate with SESTRIN2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of SESTRIN2 in mouse kidney tissue with SESTRIN2 antibody at 5 μg/ml.
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Immunofluorescence of SESTRIN2 in mouse kidney tissue with SESTRIN2 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For SESN2 (Source: Uniprot.org, NCBI)
Gene Name
SESN2
Full Name
Sestrin-2
Weight
54.494kDa
Superfamily
sestrin family
Alternative Names
DKFZp761M0212; DKFZp761M02121; Hi95; HI95sestrin-2; SES2; SEST2hypoxia induced gene 95; sestrin 2 SESN2 HI95, SES2, SEST2 sestrin 2 sestrin-2|hypoxia induced gene 95|hypoxia-induced
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SESN2, check out the SESN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SESN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SESTRIN2 SESN2 Antibody (A02558)
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No publications found for A02558
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