Product Info Summary
SKU: | A05151-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-SIRP-alpha Antibody
View all SIRP alpha/CD172a Antibodies
SKU/Catalog Number
A05151-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SIRP-alpha Antibody catalog # A05151-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SIRP-alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05151-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human SIRP-alpha1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05151-1 is reactive to Sirpa in Human, Mouse, Rat
Applications
A05151-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
56.414kDa
Background of SIRP alpha/CD172a
Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).
Furuta H., Genomics 36:206-209(1996).
Palma F., Mol. Cell. Biochem. 155:23-29(1996).
Sjoeblom T., Science 314:268-274(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Sirp alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.
Click image to see more details
Western blot analysis of various cells using SIRP-Alpha 1 Polyclonal Antibody
Protein Target Info & Infographic
Gene/Protein Information For Sirpa (Source: Uniprot.org, NCBI)
Gene Name
Sirpa
Full Name
Tyrosine-protein phosphatase non-receptor type substrate 1
Weight
56.414kDa
Alternative Names
BIT; BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; CD172a antigen; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MFRtyrosine phosphatase SHP substrate 1; MyD-1 antigen; MYD1; MYD-1; P84; protein tyrosine phosphatase, non-receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP alph Sirpa|AI835480, Bi, Bit, CD172, CD172a, P8, P84, Pt, Ptpns1, SHP, SHP-1, SHPS-1, SIRP|signal-regulatory protein alpha|tyrosine-protein phosphatase non-receptor type substrate 1|CD172 antigen-like family member A|SHP substrate 1|brain Ig-like molecule with tyrosine-based activation motifs|brain immunological-like with tyrosine-based motifs|inhibitory receptor SHPS-1|mSIRP-alpha1|myD-1 antigen|protein tyrosine phosphatase, non-receptor type substrate 1|signal-regulatory protein alpha-1|sirp-alpha-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sirpa, check out the Sirpa Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sirpa: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRP-alpha Antibody (A05151-1)
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Customer Reviews
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