Anti-SIRT5/Sirtuin 5 Antibody

Product Name

Anti-SIRT5/Sirtuin 5 Antibody

View all Sirtuin 5/SIRT5 Antibodies

SKU/Catalog Number

A02395

Size

100μl/vial

Form

Liquid

Description

Boster Bio Anti-SIRT5/Sirtuin 5 Antibody (Catalog# A02395). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat, Bovine.

Storage & Handling

Store at -80°C for long-term storage. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

Cite This Product

Anti-SIRT5/Sirtuin 5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02395)

Host

Rabbit

Contents

Liquid. In PBS.

Clonality

Polyclonal

Immunogen

Recombinant human SIRT5

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02395 is reactive to SIRT5 in Bovine, Human, Mouse, Rat

Applications

A02395 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

33.881kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1:2,500, Colorimetric, using secondary antibody coupled to alkaline phosphatase and BCIP/NBT as substrate. / 1:3,000, ECL, using secondary antibody coupled HRP). Detects a band of ~29 kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Western blot analysis of SIRT5 expression in MW marker (lane 1), recombinant human SIRT5 protein (lane 2), recombinant human SIRT3 protein (lane 3), recombinant human SIRT2 protein (lane 4) and recombinant human SIRT1 protein (lane 5). SIRT5 at 29KD was detected using rabbit anti-SIRT5 Partially purified from rabbit serum with immunodepletion polyclonal antibody (Catalog # A02395) at 1: 2500. The blot was developed using colorimetric method.

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Western blot analysis of SIRT5 expression in MW marker (lane 1), recombinant human SIRT5 protein (lane 2), recombinant human SIRT3 protein (lane 3), recombinant human SIRT2 protein (lane 4) and recombinant human SIRT1 protein (lane 5). SIRT5 at 29KD was detected using rabbit anti-SIRT5 Partially purified from rabbit serum with immunodepletion polyclonal antibody (Catalog # A02395) at 1: 3000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For SIRT5 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.5.1; EC 3.5.1.-; FLJ36950; NAD-dependent deacetylase sirtuin-5; silent mating type information regulation 2, S.cerevisiae, homolog 5; SIR2L5; sir2-like 5; SIR2-like protein 5; SIRT5; sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 5; Sirtuin 5; sirtuin type 5 SIRT5 SIR2L5 sirtuin 5 NAD-dependent protein deacylase sirtuin-5, mitochondrial|NAD-dependent deacetylase sirtuin-5|NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial|silent mating type information regulation 2, S.cerevisiae, homolog 5|sir2-like 5|sirtuin type 5

For more info on SIRT5, check out the SIRT5 Infographic

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In this infographic, you will see the following information for SIRT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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