Anti-SLP76 Purified LCP2 Monoclonal Antibody

Product Name

Anti-SLP76 Purified LCP2 Monoclonal Antibody

View all SLP-76/LCP2 Antibodies

SKU/Catalog Number

M04880

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SLP76 Purified LCP2 Monoclonal Antibody (Catalog# M04880). Tested in WB, IHC-P application(s). This antibody reacts with Pig, Human, Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-SLP76 Purified LCP2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04880)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

SLP-76/03

Isotype

Mouse IgG2b

Immunogen

Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag. The monoclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M04880 is reactive to LCP2 in Human, Mouse, Pig

Applications

M04880 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

60.188kDa

Background of SLP-76/LCP2

SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): 10 μg/ml; positive tissue: thymus

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-SLP76 Purified LCP2 Monoclonal Antibody M04880

Western blotting analysis (reducing conditions) of whole cell lysate using anti-SLP76 (SLP-76/03).
Lane 1: HPB-ALL human peripheral blood T cell leukemia cell line
Lane 2: JURKAT human peripheral blood T cell leukemia cell line
Lane 3: RAMOS human Burkitt lymphoma cell line
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For LCP2 (Source: Uniprot.org, NCBI)

Alternative Names

LCP2; lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of76kDa); lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of76kD); lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SH2 domain-containing leukocyte protein of 76kD; SLP-76 tyrosine phosphoprotein; SLP76; SLP-76; SLP7676 kDa tyrosine phosphoprotein LCP2 SLP-76, SLP76 lymphocyte cytosolic protein 2 lymphocyte cytosolic protein 2|76 kDa tyrosine phosphoprotein|SH2 domain-containing leukocyte protein of 76 kDa|SLP-76 tyrosine phosphoprotein

For more info on LCP2, check out the LCP2 Infographic

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In this infographic, you will see the following information for LCP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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