Anti-Smac/Diablo Antibody

Product Name

Anti-Smac/Diablo Antibody

View all SMAC/Diablo Antibodies

SKU/Catalog Number

A03790

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Smac/Diablo Antibody (Catalog # A03790). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Smac/Diablo Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03790)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This whole rabbit serum was prepared by repeated immunizations with recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03790 is reactive to DIABLO in Human

Applications

A03790 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

27.131kDa

Background of SMAC/Diablo

Apoptosis is a conserved cell suicide program essential for the development and homeostasis of multi-cellular organisms. Abnormal inhibition of apoptosis is a hallmark of cancer and autoimmune diseases, whereas excessive cell death is found in neurodegenerative disorders such as Alzheimers disease. Executioners of the apoptotic program are cysteine proteases termed caspases that exist as inactive zymogens in living cells and are activated during apoptosis. Active caspases cleave key intracellular protein substrates, resulting in the characteristic morphological changes associated with apoptosis. The release of cytochrome c from the mitochondria triggers the oligomerization of Apaf-1 in an ATP/dATP-dependent manner and induces the autoactivation of caspase-9.  Active caspase-9 in turn activates downstream effector caspases including caspase -3, -6 and -7.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
IP: 1:100
WB: 1:1,000 - 1:2,000

Validation Images & Assay Conditions

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Western blot analysis using rabbit anti-Smac polyclonal antibody (Catalog # A03790) at 1:1000 detected a 25-26 kDa band in partially purified recombinant human Smac protein. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

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Western blot using rabbit anti-Smac polyclonal antibody (Catalog # A03790) at 1:1000 detects a 26 kDa band when 1

Gene/Protein Information For DIABLO (Source: Uniprot.org, NCBI)

Alternative Names

0610041G12Rik; Diablo; diablo, IAP-binding mitochondrial protein; Direct IAP-binding protein with low pI; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMACmitochondrial DIABLO DFNA64, SMAC diablo IAP-binding mitochondrial protein diablo homolog, mitochondrial|diablo-like protein|direct IAP-binding protein with low pI|second mitochondria-derived activator of caspase

For more info on DIABLO, check out the DIABLO Infographic

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In this infographic, you will see the following information for DIABLO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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