Anti-Smad1 (phospho-S187) Antibody

Product Name

Anti-Smad1 (phospho-S187) Antibody

View all SMAD1 Antibodies

SKU/Catalog Number

A00728S187

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Smad1 (phospho-S187) Antibody catalog # A00728S187. Tested in WB,IHC,ICC,IP applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Smad1 (phospho-S187) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00728S187)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human BLM around the phosphorylation site of T99.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00728S187 is reactive to SMAD1 in Human, Mouse, Rat

Applications

A00728S187 is guaranteed for IP, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

52.26kDa

Background of SMAD1

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

Ana V. Miletic, J. Biol. Chem., Dec 2006; 281: 38257 - 38265.
Christopher C. Stebbins, Mol. Cell. Biol., Sep 2003; 23: 6291 - 6299.
Vered Schapira, Cancer Res., Jun 2006; 66: 6183 - 6191.
Maya Groysman, J. Biol. Chem., Dec 2002; 277: 50121 - 50130.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of SMAD1 using Anti-Smad1 (phospho-S187) Antibody (A00728S187).

Immunohistochemistry (IHC) analyzes of p-Smad1 (S187) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody

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Figure 1. Western blotting validation for Anti-Smad1 (phospho-S187) Antibody A00728S187

Western blot (WB) analysis of p-Smad1 (S187) polyclonal antibody at 1:500 dilution
Lane1:Hela cell lysate treated with UV(4h)
Lane2:Raw264.7 cell lysate treated with UV(4h)
Lane3:H9C2 cell lysate treated with UV(4h)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For SMAD1 (Source: Uniprot.org, NCBI)

Alternative Names

BSP1; BSP-1; hSMAD1; JV4-1SMAD, mothers against DPP homolog 1 (Drosophila); MAD homolog 1; MADH1JV41; MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila); Mad-related protein 1; mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; SMAD family member 1SMAD 1; SMAD, mothers against DPP homolog 1; Smad1; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1 SMAD1 BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1 SMAD family member 1 mothers against decapentaplegic homolog 1|MAD, mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD, mothers against DPP homolog 1|TGF-beta signaling protein 1|mothers against DPP homolog 1|transforming growth factor-beta signaling protein 1

For more info on SMAD1, check out the SMAD1 Infographic

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In this infographic, you will see the following information for SMAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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