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Product Info Summary
| SKU: | M00238 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SOD1/Cu Zn Sod Rabbit Monoclonal Antibody
View all SOD1/Cu-Zn SOD Antibodies
SKU/Catalog Number
M00238
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SOD1/Cu Zn Sod Rabbit Monoclonal Antibody catalog # M00238. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOD1/Cu Zn Sod Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00238)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOEI-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SOD1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00238 is reactive to SOD1 in Human, Mouse, Rat
Applications
M00238 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
100-110 kDa
Calculated molecular weight
15.936kDa
Background of SOD1/Cu-Zn SOD
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of CD80 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For SOD1 (Source: Uniprot.org, NCBI)
Gene Name
SOD1
Full Name
Superoxide dismutase [Cu-Zn]
Weight
15.936kDa
Superfamily
Cu-Zn superoxide dismutase family
Alternative Names
ALS; ALS1; amyotrophic lateral sclerosis 1 (adult); Cu; Cu/Zn superoxide dismutase; CuZn SOD; Cu-Zn SOD; EC 1.15.1.1; homodimer; hSod1; indophenoloxidase A; Ipo1; IPOA; SOD; SOD, cytosolic; SOD, Soluble; SOD1; superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; superoxide dismutase 1, soluble; Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic SOD1 ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer superoxide dismutase 1 superoxide dismutase [Cu-Zn]|Cu/Zn superoxide dismutase|SOD, soluble|epididymis secretory protein Li 44|indophenoloxidase A|superoxide dismutase 1, soluble|superoxide dismutase, cystolic
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOD1, check out the SOD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOD1/Cu Zn Sod Rabbit Monoclonal Antibody (M00238)
Hello CJ!
M00238 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Akt specific activator SC79 protects against early brain injury following subarachnoid hemorrhage
Protective Role of Antioxidant Huskless Barley Extracts on TNF-?-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells
Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation
Guo Z, Qi W, Yu Y, Du S, Wu J, Liu J. Diabetol Metab Syndr. 2014 Feb 28;6(1):29. Doi: 10.1186/1758-5996-6-29. Effect Of Exenatide On The Cardiac Expression Of Adiponectin Receptor 1 And Nadph Oxidase Subunits And Heart Function In Streptozotocin-I...
Hou S, Zheng F, Li Y, Gao L, Zhang J. Int J Mol Sci. 2014 Aug 26;15(9):15026-43. Doi: 10.3390/Ijms150915026. The Protective Effect Of Glycyrrhizic Acid On Renal Tubular Epithelial Cell Injury Induced By High Glucose.
Long Sh, Yu Zq, Shuai L, Guo Yl, Duan Dl, Xu Xy, Li Xd. Int J Mol Sci. 2012;13(3):3354-65. Doi: 10.3390/Ijms13033354. Epub 2012 Mar 12. The Hypoglycemic Effect Of The Kelp On Diabetes Mellitus Model Induced By Alloxan In Rats.
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-SOD1/Cu Zn Sod Rabbit Monoclonal Antibody
Question
We are currently using anti-SOD1/Cu Zn Sod Rabbit Monoclonal antibody M00238 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on primate tissues as well?
T. Brown
Verified customer
Asked: 2019-08-28
Answer
The anti-SOD1/Cu Zn Sod Rabbit Monoclonal antibody (M00238) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-28
Question
you antibody using your anti-SOD1/Cu Zn Sod Rabbit Monoclonal antibody for sensory perception of sound studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
We appreciate your inquiry. This M00238 anti-SOD1/Cu Zn Sod Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for IF, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-13
Question
We have seen staining in human fetal brain cortex. Any tips? Is anti-SOD1/Cu Zn Sod Rabbit Monoclonal antibody supposed to stain fetal brain cortex positively?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
Based on literature fetal brain cortex does express SOD1. Based on Uniprot.org, SOD1 is expressed in pons, colon, placenta, fetal brain cortex, cervix carcinoma, liver, among other tissues. Regarding which tissues have SOD1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Colon, Pubmed ID: 14702039
Fetal brain cortex, Pubmed ID: 8528216
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-01
Question
My colleagues were happy with the WB result of your anti-SOD1/Cu Zn Sod Rabbit Monoclonal antibody. However we have been able to see positive staining in pons cytoplasm. using this antibody. Is that expected? Could you tell me where is SOD1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-08-07
Answer
According to literature, pons does express SOD1. Generally SOD1 expresses in cytoplasm. Regarding which tissues have SOD1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Colon, Pubmed ID: 14702039
Fetal brain cortex, Pubmed ID: 8528216
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-08-07
Question
We purchased anti-SOD1/Cu Zn Sod Rabbit Monoclonal antibody for IF on pons a few years ago. I am using rat, and I plan to use the antibody for WB next. My lab would like examining pons as well as fetal brain cortex in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-06-29
Answer
I have checked the website and datasheets of our anti-SOD1/Cu Zn Sod Rabbit Monoclonal antibody and it appears that M00238 has been validated on rat in both IF and WB. Thus M00238 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-06-29
