Product Info Summary
SKU: | A15765 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-TCR beta Antibody
SKU/Catalog Number
A15765
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TCR beta Antibody catalog # A15765. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCR beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15765)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human C18orf10
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15765 is reactive to TRBC1 in Human
Applications
A15765 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
19.769kDa
Background of TRBC1
BIVM (for basic, immunoglobulin-like variable motif-containing) refers to a recently identified gene product that maps to human chromosome 13q32-q33 and is predicted to encode a 503-amino acid protein. BIVM shows ubiquitous expression in normal human tissue and the presence of a 5' CpG island suggests it is a housekeeping gene. BIVM is likely essential for some aspect of basic cellular function. BIVM is highly charged and localizes to the cytoplasm and nucleus where it may bind to either DNA or RNA or associate with other cellular proteins. Significant sequence homology exists with many organisms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of various cells using TCR Beta Polyclonal Antibody
Click image to see more details
Western blot analysis of lysate from COLO205 cells treated with Forskolin, using TCR Beta antibody.
Protein Target Info & Infographic
Gene/Protein Information For TRBC1 (Source: Uniprot.org, NCBI)
Gene Name
TRBC1
Full Name
T cell receptor beta constant 1
Weight
19.769kDa
Alternative Names
BV05S1J2.2; TCRBC1; TRBC1 T cell receptor beta constant 1 TRBC1 BV05S1J2.2, TCRB, TCRBC1 T cell receptor beta constant 1 V_segment translation product
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRBC1, check out the TRBC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRBC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TCR beta Antibody (A15765)
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No publications found for A15765
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-TCR beta Antibody
Question
Will anti-TCR beta antibody A15765 work on monkey WB with thymus?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
Our lab technicians have not validated anti-TCR beta antibody A15765 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-TCR beta antibody A15765 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey thymus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-30
Question
We want to test anti-TCR beta antibody A15765 on human thymus for research purposes, then I may be interested in using anti-TCR beta antibody A15765 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The products we sell, including anti-TCR beta antibody A15765, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-24
Question
I was wanting to use your anti-TCR beta antibody for WB for human thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human thymus identification?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
As indicated on the product datasheet, A15765 anti-TCR beta antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-02
Question
We are currently using anti-TCR beta antibody A15765 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The anti-TCR beta antibody (A15765) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-19
Question
I have a question about product A15765, anti-TCR beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Banerjee
Verified customer
Asked: 2013-02-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15765 anti-TCR beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-02-05