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Product Info Summary
| SKU: | A15765 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TCR beta Antibody
SKU/Catalog Number
A15765
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TCR beta Antibody catalog # A15765. Tested in WB, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCR beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15765)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TCR beta. AA range:66-115
Reactive Species
A15765 is reactive to TRBC1 in Human
Calculated molecular weight
19.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15765 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using TCR β Polyclonal Antibody
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Western blot analysis of lysate from COLO205 cells treated with Forskolin, using TCR β antibody.
Specific Publications For Anti-TCR beta Antibody (A15765)
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Customer Q&As
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5 Customer Q&As for Anti-TCR beta Antibody
Question
Will anti-TCR beta antibody A15765 work on monkey WB with thymus?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
Our lab technicians have not validated anti-TCR beta antibody A15765 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-TCR beta antibody A15765 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey thymus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-30
Question
We want to test anti-TCR beta antibody A15765 on human thymus for research purposes, then I may be interested in using anti-TCR beta antibody A15765 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The products we sell, including anti-TCR beta antibody A15765, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-24
Question
I was wanting to use your anti-TCR beta antibody for WB for human thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human thymus identification?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
As indicated on the product datasheet, A15765 anti-TCR beta antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-02
Question
We are currently using anti-TCR beta antibody A15765 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The anti-TCR beta antibody (A15765) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-19
Question
I have a question about product A15765, anti-TCR beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Banerjee
Verified customer
Asked: 2013-02-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15765 anti-TCR beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-02-05
