Anti-TGF beta 1 TGFB1 Antibody

Product Name

Anti-TGF beta 1 TGFB1 Antibody

View all TGFB1 Antibodies

SKU/Catalog Number

A00019

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-TGF beta 1 TGFB1 Antibody (Catalog # A00019). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-TGF beta 1 TGFB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00019)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Clone Number

WH-3

Isotype

IgG

Immunogen

TGF beta 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of the mature growth factor (112 amino acids in length).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00019 is reactive to TGFB1 in Human

Applications

A00019 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

44.341kDa

Background of TGFB1

TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, including three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule; the amino acid sequence between human and mouse differs by only one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues, such as placenta and kidneys.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:20,000
IF Microscopy: User optimized
WB: 1:100 - 1:500

Validation Images & Assay Conditions

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Western blot analysis of TGF beta 1 in reduced recombinant TGF Beta 1 (lane 1), ladder (lane 2) and Non-reduced recombinant TGF Beta 1 (lane 3) using rabbit anti-TGF beta 1 affinity purified polyclonal antibody (Catalog # A00019) at 1:200. Load: 250 ng per lane. Predicted/Observed size: 12 kDa/25 kDa, 12 kDa for reduced TGF Beta 1/25 kDa for non-reduced TGF Beta 1. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

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ELISA results of rabbit anti-TGF beta 1 affinity purified polyclonal antibody (Catalog # A00019) tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1

Gene/Protein Information For TGFB1 (Source: Uniprot.org, NCBI)

Alternative Names

CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1 TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1

For more info on TGFB1, check out the TGFB1 Infographic

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In this infographic, you will see the following information for TGFB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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