Product Info Summary
SKU: | A01871-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TMEM173 Antibody
SKU/Catalog Number
A01871-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TMEM173 Antibody catalog # A01871-2. Tested in WB,Flow Cytometry,ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TMEM173 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01871-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RAD21.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01871-2 is reactive to PYCARD in Human
Applications
A01871-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
21.627kDa
Background of ASC/TMS1
Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
McKay M.J., Genomics 36:305-315(1996).
Sadano H., Biochem. Biophys. Res. Commun. 267:418-422(2000).
Nomura N., DNA Res. 1:223-229(1994).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
FC: 1:50-1:100
ICC/IF: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Flow Cytometry validation of TMEM173 using Anti-TMEM173 Antibody (A01871-2).
Flow cytometric analysis of THP-1 cells with TMEM173 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Click image to see more details
Figure 1. Western blotting validation for Anti-TMEM173 Antibody A01871-2
Western blot analysis of TMEM173 on U937 (1) and 293 (2) cell lysate using anti-TMEM173 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For PYCARD (Source: Uniprot.org, NCBI)
Gene Name
PYCARD
Full Name
Apoptosis-associated speck-like protein containing a CARD
Weight
21.627kDa
Alternative Names
ASC; ASCMGC10332; CARD5; CARD5TMS; caspase recruitment domain protein 5; Caspase recruitment domain-containing protein 5; hASC; PYCARD; PYD and CARD domain containing; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1; TMS1; TMS-1; TMS1apoptosis-associated speck-like protein containing a CARD PYCARD ASC, CARD5, TMS, TMS-1, TMS1 PYD and CARD domain containing apoptosis-associated speck-like protein containing a CARD|caspase recruitment domain-containing protein 5|target of methylation-induced silencing 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PYCARD, check out the PYCARD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PYCARD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMEM173 Antibody (A01871-2)
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No publications found for A01871-2
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