Anti-TNF Receptor I/TNFRSF1A Antibody

TNF RI/TNFRSF1A antibody

Boster Bio Anti-TNF Receptor I/TNFRSF1A Antibody catalog # PA1210. Tested in IHC-P, WB applications. This antibody reacts with Human. Cited in 5 publication(s).

Product Info Summary

SKU: PA1210
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC-P, WB

Product Name

Anti-TNF Receptor I/TNFRSF1A Antibody

View all TNF RI/TNFRSF1A Antibodies

SKU/Catalog Number

PA1210

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TNF Receptor I/TNFRSF1A Antibody catalog # PA1210. Tested in IHC-P, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TNF Receptor I/TNFRSF1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1210)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human TNF Receptor I (195-211aa CLPQIENVKGTEDSGTT) .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1210 is reactive to TNFRSF1A in Human

Applications

PA1210 is guaranteed for IHC-P, WB Boster Guarantee

Background of TNF RI/TNFRSF1A

Tumor necrosis factor receptor 1 (TNFR1), a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. Its gene is located on 12p13.2. The coding region and the 3-prime untranslated region of TNFR1 are distributed over 10 exons. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. Additionally, TNFR1 associates with the MADD protein through a death domain-death domain interaction. MADD provides a physical link between TNFR1 and the induction of mitogen-activated protein (MAP) kinase (e.g., ERK2) activation and arachidonic acid release. TNFR1-induced apoptosis involves 2 sequential signaling complexes. Complex I, the initial plasma membrane-bound complex, consists of TNFR1, the adaptor TRADD, the kinase RIP1, and TRAF2 and rapidly signals activation of NF-kappa-B. In a second step, TRADD and RIP1 associate with FADD and caspase-8, forming a cytoplasmic complex, complex II.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For TNFRSF1A (Source: Uniprot.Org, NCBI)

Uniprot ID

P19438

Gene ID

7132

Gene Name

TNFRSF1A

Full Name

Tumor necrosis factor receptor superfamily member 1A

Weight

50.495kDa

Alternative Names

CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor TNFRSF1A CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60 TNF receptor superfamily member 1A tumor necrosis factor receptor superfamily member 1A|TNF-R1|TNF-RI|TNFR-I|tumor necrosis factor binding protein 1|tumor necrosis factor receptor type 1|tumor necrosis factor-alpha receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFRSF1A, check out the TNFRSF1A Infographic

TNFRSF1A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFRSF1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1210 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cytokine expression associated with Helicobacter pylori and Epstein‐Barr virus infection in gastric carcinogenesis

Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir

Soluble Tumor Necrosis Factor Receptor Mediates Cell Proliferation on Lipopolysaccharide-Stimulated Cultured Human Decidual Stromal Cells

TRAF6 upregulation in spinal astrocytes maintains neuropathic pain by integrating TNF-? and IL-1? signaling

Powerful inner/outer controlled multi-target magnetic nanoparticle drug carrier prepared by liquid photo-immobilization

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5 Customer Q&As for Anti-TNF Receptor I/TNFRSF1A Antibody

Question

In the Anti-TNF Receptor I/TNFRSF1A Antibody (PA1210) protocol, the optimal concentration for the primary antibody is 1 μg/ 1 ml and the primary antibody is sold 100 μg/vial, so does that mean that this primary antibody is enough for 100 slides?

Verified customer

Asked: 2022-05-12

Answer

For working concentration of 1ug/ml, 100ug is enough for around 1000 slides. 1ml is enough for around 10 slides.

Boster Scientific Support

Answered: 2022-05-13

Question

Why do I get a band on WB at 75 kD and at 25kD?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

The additional band at 75kD has been identified as glycosylated TNFR1.

Boster Scientific Support

Answered: 2020-01-24

Question

See below the WB image, lot number and protocol we used for tongue uterus using anti-TNF Receptor I/TNFRSF1A antibody PA1210. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-21

Question

I was wanting to use your anti-TNF Receptor I/TNFRSF1A antibody for IHC-P for human tongue uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tongue uterus identification?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

It shows on the product datasheet, PA1210 anti-TNF Receptor I/TNFRSF1A antibody has been validated for IHC-P, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tongue uterus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-29

Question

We are currently using anti-TNF Receptor I/TNFRSF1A antibody PA1210 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

The anti-TNF Receptor I/TNFRSF1A antibody (PA1210) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-21

Size

Total: $315

SKU:PA1210

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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