Overview
| Product Name |
Anti-TNF Receptor I/TNFRSF1A Antibody
See more TNF RI/TNFRSF1A products |
| Catalog# |
PA1210 |
| Pack Size |
100μg/vial |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-TNF Receptor I/TNFRSF1A Antibody catalog # PA1210. Tested in IHC-P, WB applications. This antibody reacts with Human. |
| Cite This Product |
Anti-TNF Receptor I/TNFRSF1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1210)
|
| Antibodies Validation |
Antibodies Validation Information
|
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Anti-TNF Receptor I/TNFRSF1A Antibody may replace the following items: sc 1067|sc 1068|sc 1069|sc 1163|sc 12746|sc 130220|sc 271031|sc 271031 X|sc 31349|sc 31350|sc 31351|sc 376453|sc 376453 X|sc 390368|sc 390368 X|sc 46653|sc 48847|sc 48847 X|sc 48848|sc 48848 X|sc 48849|sc 48849 X|sc 52739|sc 7868|sc 7895|sc 8436|sc 98896|sc 98896 X. |
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human |
| Applications |
IHC-P, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $100. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
A synthetic peptide corresponding to a sequence in the middle region of human TNF Receptor I (195-211aa CLPQIENVKGTEDSGTT) . |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For TNFRSF1A (Source: Uniprot.org, NCBI)
| Uniprot Id | P19438 |
|---|
| NCBI Gene Id | 7132 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | TNFRSF1A |
|---|
| Protein Name | Tumor necrosis factor receptor superfamily member 1A |
|---|
| Alternative Names | TNF RI/TNFRSF1A|CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor |
|---|
| Molecular Weight | 50495 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TNFRSF1A, check out the TNFRSF1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for TNFRSF1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the TNFRSF1A infographic
Our Boster Quality Guarantee for Anti-TNF Receptor I/TNFRSF1A Antibody covers its use in the following applications.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti-TNF Receptor I antibody, PA1210, IHC(P)
IHC(P): Human Mammary Tissue
Figure 1. Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (PA1210).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW579 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # PA1210) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50-60KD. The expected band size for TNF Receptor I is at 50KD.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
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PubMed ID 19564935 |
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PubMed ID 25267210 |
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Questions and answers from customers related to PA1210 Anti-TNF Receptor I/TNFRSF1A Antibody
4 Related Questions
Question
Why do I get a band on WB at 75 kD and at 25kD?
Verified Customer
Asked: 2020-01-24
Answer
The additional band at 75kD has been identified as glycosylated TNFR1.
Boster Scientific Support
Answered: 2020-01-24
Question
See below the WB image, lot number and protocol we used for tongue uterus using anti-TNF Receptor I/TNFRSF1A antibody PA1210. Please let me know if you require anything else.
Verified Customer
Asked: 2019-11-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-21
Question
I was wanting to use your anti-TNF Receptor I/TNFRSF1A antibody for IHC-P for human tongue uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tongue uterus identification?
Verified Customer
Asked: 2019-08-29
Answer
It shows on the product datasheet, PA1210 anti-TNF Receptor I/TNFRSF1A antibody has been validated for IHC-P, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tongue uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-29
Question
We are currently using anti-TNF Receptor I/TNFRSF1A antibody PA1210 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Asked: 2019-05-21
Answer
The anti-TNF Receptor I/TNFRSF1A antibody (PA1210) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-21
