Anti-TNFC LTB Antibody

Lymphotoxin beta/TNFSF3 antibody

Boster Bio Anti-TNFC LTB Antibody (Catalog# A30476). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A30476
Size: 100ul
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IF, ICC, WB

Product Name

Anti-TNFC LTB Antibody

View all Lymphotoxin beta/TNFSF3 Antibodies

SKU/Catalog Number

A30476

Size

100ul

Form

Liquid

Description

Boster Bio Anti-TNFC LTB Antibody (Catalog# A30476). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TNFC LTB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30476)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human TNFC. Immunogen sequence location: 181-230

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30476 is reactive to LTB in Human, Mouse

Applications

A30476 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

25.39kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For LTB (Source: Uniprot.org, NCBI)

Gene Name

LTB

Full Name

Lymphotoxin-beta

Weight

25.39kDa

Superfamily

tumor necrosis factor family

Alternative Names

LTB; lymphotoxin beta (TNF superfamily, member 3); Lymphotoxin beta; lymphotoxin-beta; p33; TNFCTNF-C; TNFSF3; TNFSF3LT-beta; Tumor necrosis factor C; Tumor necrosis factor ligand superfamily member 3 LTB TNFC, TNFSF3, TNLG1C, p33 lymphotoxin beta lymphotoxin-beta|LT-beta|TNF superfamily member 3|TNF-C|lymphotoxin beta (TNF superfamily, member 3)|tumor necrosis factor C|tumor necrosis factor ligand 1C|tumor necrosis factor ligand superfamily member 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LTB, check out the LTB Infographic

LTB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-TNFC LTB Antibody

Question

Does A30476 anti-TNFC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

As indicated on the product datasheet, A30476 anti-TNFC antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-03

Question

We are currently using anti-TNFC antibody A30476 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

The anti-TNFC antibody (A30476) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-04

Question

See attached the WB image, lot number and protocol we used for spleen using anti-TNFC antibody A30476. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-27

Question

I was wanting to use your anti-TNFC antibody for WB for human spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human spleen identification?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

As indicated on the product datasheet, A30476 anti-TNFC antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-12

Question

Do you have a BSA free version of anti-TNFC antibody A30476 available?

Verified Customer

Verified customer

Asked: 2018-08-31

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TNFC antibody A30476 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-08-31

Question

Would anti-TNFC antibody A30476 work on dog WB with colon?

L. Mitchell

Verified customer

Asked: 2014-06-04

Answer

Our lab technicians have not tested anti-TNFC antibody A30476 on dog. You can run a BLAST between dog and the immunogen sequence of anti-TNFC antibody A30476 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog colon in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-06-04

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-TNFC antibody A30476. Let me know if you need anything else.

K. Carter

Verified customer

Asked: 2013-09-11

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-09-11

Order DetailsPrice
A30476

100 μL

$335

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A30476
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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