Click image to see more details
Product Info Summary
| SKU: | A02009-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TWEAK/Tnfsf12 Antibody Picoband™
View all TWEAK/TNFSF12 Antibodies
SKU/Catalog Number
A02009-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TWEAK/Tnfsf12 Antibody Picoband™ catalog # A02009-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TWEAK/Tnfsf12 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02009-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse TWEAK/Tnfsf12 recombinant protein (Position: S46-H249).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02009-3 is reactive to TNFSF12 in Mouse, Rat
Applications
A02009-3 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
27 kDa
Calculated molecular weight
27.216kDa
Background of TWEAK/TNFSF12
Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis (TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. Predicted to enable receptor ligand activity. Acts upstream of or within extrinsic apoptotic signaling pathway. Located in extracellular region. Is expressed in Meckel's cartilage; hip; skeleton; and superior cervical ganglion. Orthologous to several human genes including TNFSF12-TNFSF13 (TNFSF12-TNFSF13 readthrough).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TWEAK/Tnfsf12 using anti-TWEAK/Tnfsf12 antibody (A02009-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWEAK/Tnfsf12 antigen affinity purified polyclonal antibody (Catalog # A02009-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWEAK/Tnfsf12 at approximately 27 kDa. The expected band size for TWEAK/Tnfsf12 is at 22 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TNFSF12 (Source: Uniprot.org, NCBI)
Gene Name
TNFSF12
Full Name
Tumor necrosis factor ligand superfamily member 12
Weight
27.216kDa
Superfamily
tumor necrosis factor family
Alternative Names
APO3 ligand; APO3LMGC20669; DR3LGTWEAKAPO3/DR3 ligand; MGC129581; TNF-related weak inducer of apoptosis; TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TWEAK TNFSF12 APO3L, DR3LG, TNLG4A, TWEAK TNF superfamily member 12 tumor necrosis factor ligand superfamily member 12|APO3 ligand|APO3/DR3 ligand|TNF-related WEAK inducer of apoptosis|tumor necrosis factor (ligand) superfamily, member 12|tumor necrosis factor ligand 4A|tumor necrosis factor superfamily member 12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFSF12, check out the TNFSF12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFSF12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TWEAK/Tnfsf12 Antibody Picoband™ (A02009-3)
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