Anti-Tyrosine-protein kinase BTK BTK Antibody

Product Name

Anti-Tyrosine-protein kinase BTK BTK Antibody

View all BTK Antibodies

SKU/Catalog Number

A00245

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Tyrosine-protein kinase BTK BTK Antibody (Catalog # A00245). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

BTK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Tyrosine-protein kinase BTK BTK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00245)

Host

Rabbit

Contents

BTK Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK. The immunogen is located within amino acids 20 - 70 of BTK.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Multiple isoforms of BTK are known to exist.

Reactive Species

A00245 is reactive to BTK in Human, Mouse

Applications

A00245 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

76.281kDa

Background of BTK

Bruton's tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked agamma-globulinaemia, and has since been shown to involved in a number of signaling pathways in hemapoietic lineage. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

BTK antibody can be used for the detection of BTK by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of BTK in U937 cell lysate with BTK antibody at (A) 0.5

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Immunohistochemistry of BTK in mouse brain tissue with BTK antibody at 5 μg/mL.

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Immunofluorescence of BTK in Daudi cells with BTK antibody at 10 μg/mL.

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Immunocytochemistry of BTK in Daudi cells with BTK antibody at 10 μg/mL.

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Immunofluorescence of BTK in mouse brain tissue with BTK antibody at 20 μg/ml.

Red: BTK Antibody (A00245)
Blue: DAPI staining

Gene/Protein Information For BTK (Source: Uniprot.org, NCBI)

Alternative Names

Agammaglobulinaemia tyrosine kinase; AGMX1; AGMX1MGC126262; AT; ATKIMD1; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; BTK; dominant-negative kinase-deficient Brutons tyrosine kinase; EC 2.7.10; EC 2.7.10.2; IMD1; PSCTK1; tyrosine-protein kinase BTK; XLA; XLAMGC126261 BTK AGMX1, AT, ATK, BPK, IGHD3, IMD1, PSCTK1, XLA Bruton tyrosine kinase tyrosine-protein kinase BTK|B-cell progenitor kinase|Bruton agammaglobulinemia tyrosine kinase|Brutons tyrosine kinase|agammaglobulinaemia tyrosine kinase|dominant-negative kinase-deficient Brutons tyrosine kinase|truncated Bruton agammaglobulinemia tyrosine kinase

For more info on BTK, check out the BTK Infographic

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In this infographic, you will see the following information for BTK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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