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Product Info Summary
| SKU: | PA1420 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Ubiquitin/UBB Antibody
SKU/Catalog Number
PA1420
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ubiquitin/UBB Antibody catalog # PA1420. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ubiquitin/UBB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1420)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Ubiquitin, identical to the rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1420 is reactive to UBB in Human, Mouse, Rat
Applications
PA1420 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
160-190 kDa
Calculated molecular weight
25.762kDa
Background of UBB
Ubiquitin (originally, ubiquitous immunopoietic polypeptide) is a small regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. Its gene is mapped to 16p 13.3. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Ubiquitin binds to proteins and labels them for destruction. It directs protein recycling and also direct proteins to other locations in the cell, where they control other protein and cell mechanisms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-Ubiquitin antibody, PA1420, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Human MCF-7 Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: SMMC Cell Lysate
Click image to see more details
Anti-Ubiquitin antibody, PA1420, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For UBB (Source: Uniprot.org, NCBI)
Gene Name
UBB
Full Name
Polyubiquitin-B
Weight
25.762kDa
Superfamily
ubiquitin family
Alternative Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin UBB HEL-S-50 ubiquitin B polyubiquitin-B|epididymis secretory protein Li 50|polyubiquitin B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBB, check out the UBB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ubiquitin/UBB Antibody (PA1420)
Hello CJ!
PA1420 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang DY,Mou YN,Du X,Guan Y,Feng MG. Ubr1-mediated ubiquitylation orchestrates asexual development, polar growth, and virulence-related cellular events in Beauveria bassiana. Appl Microbiol Biotechnol.2021 Mar 8.doi:10.1007/s00253-021-11222-0.Epub ahead of print.PMID:33686455.
Species: B. bassiana strain
PA1420 usage in article: APP:WB, SAMPLE:CELL CULTURE, DILUTION:1:2000
Rong H,Wang C,Liu H,Zhang M,Yuan Y,Pu Y,Huang J,Yu J. Biochemical Toxicity and Potential Detoxification Mechanisms in Earthworms Eisenia fetida Exposed to Sulfamethazine and Copper. Bull Environ Contam Toxicol.2020 Aug;105(2):255-260. doi:10.1007/s00128-0
Species: Earthworm
PA1420 usage in article: APP:WB, SAMPLE:EARTHWORM HOMOGENATES, DILUTION:1:3000
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-Ubiquitin/UBB Antibody
Question
Can you help my question with product PA1420, anti-Ubiquitin/UBB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Wu
Verified customer
Asked: 2020-02-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1420 anti-Ubiquitin/UBB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-24
Question
Is this PA1420 anti-Ubiquitin/UBB antibody reactive to the isotypes of UBB?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The immunogen of PA1420 anti-Ubiquitin/UBB antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Ubiquitin(29-45aa KIQDKEGIPPDQQRLIF), identical to the rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-15
Question
Our team were happy with the WB result of your anti-Ubiquitin/UBB antibody. However we have observed positive staining in liver lung ubiquitin: cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is UBB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
From what I have seen in literature, liver lung does express UBB. Generally UBB expresses in ubiquitin: cytoplasm. nucleus. Regarding which tissues have UBB expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3029682
Brain, Liver, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 1128706
Boster Scientific Support
Answered: 2020-01-15
Question
I was wanting to use your anti-Ubiquitin/UBB antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
It shows on the product datasheet, PA1420 anti-Ubiquitin/UBB antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-19
Question
Would PA1420 anti-Ubiquitin/UBB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Krishna
Verified customer
Asked: 2019-12-03
Answer
It shows on the product datasheet, PA1420 anti-Ubiquitin/UBB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-03
Question
My question regards to test anti-Ubiquitin/UBB antibody PA1420 on rat brain for research purposes, then I may be interested in using anti-Ubiquitin/UBB antibody PA1420 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The products we sell, including anti-Ubiquitin/UBB antibody PA1420, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-12
Question
We have observed staining in mouse hypothalamus. What should we do? Is anti-Ubiquitin/UBB antibody supposed to stain hypothalamus positively?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
Based on literature hypothalamus does express UBB. Based on Uniprot.org, UBB is expressed in hypothalamus, blood, brain, liver lung, fetal brain cortex, among other tissues. Regarding which tissues have UBB expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3029682
Brain, Liver, and Lung, Pubmed ID: 15489334
Fetal brain cortex, Pubmed ID: 1128706
Boster Scientific Support
Answered: 2019-10-08
Question
Is a blocking peptide available for product anti-Ubiquitin/UBB antibody (PA1420)?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
We do provide the blocking peptide for product anti-Ubiquitin/UBB antibody (PA1420). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-19
Question
Would anti-Ubiquitin/UBB antibody PA1420 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
According to the expression profile of brain, UBB is highly expressed in brain. So, it is likely that anti-Ubiquitin/UBB antibody PA1420 will work for WB with brain.
Boster Scientific Support
Answered: 2019-08-28
Question
Is there a BSA free version of anti-Ubiquitin/UBB antibody PA1420 available?
Verified Customer
Verified customer
Asked: 2019-01-09
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Ubiquitin/UBB antibody PA1420 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-09
Question
My lab would like using your anti-Ubiquitin/UBB antibody for i-kappab kinase/nf-kappab signaling studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
H. Mitchell
Verified customer
Asked: 2018-01-24
Answer
Thanks for your inquiry. This PA1420 anti-Ubiquitin/UBB antibody is validated on rat thymus tissue, tissue lysate, mm231 cell lysate, hela cell lysate, smmc cell lysate, lung cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-01-24
Question
Would anti-Ubiquitin/UBB antibody PA1420 work on feline WB with fetal brain cortex?
O. Gonzalez
Verified customer
Asked: 2017-02-28
Answer
Our lab technicians have not tested anti-Ubiquitin/UBB antibody PA1420 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Ubiquitin/UBB antibody PA1420 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline fetal brain cortex in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-28
Question
Here is the WB image, lot number and protocol we used for brain using anti-Ubiquitin/UBB antibody PA1420. Please let me know if you require anything else.
J. Singh
Verified customer
Asked: 2016-05-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-10
Question
We are currently using anti-Ubiquitin/UBB antibody PA1420 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
S. Collins
Verified customer
Asked: 2014-12-31
Answer
The anti-Ubiquitin/UBB antibody (PA1420) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Ubiquitin/UBB antibody PA1420. Let me know if you need anything else.
F. Anderson
Verified customer
Asked: 2014-07-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-28
Question
I see that the anti-Ubiquitin/UBB antibody PA1420 works with WB, what is the protocol used to produce the result images on the product page?
T. Miller
Verified customer
Asked: 2014-07-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-07-02
Question
We bought anti-Ubiquitin/UBB antibody for WB on liver lung in the past. I am using rat, and I plan to use the antibody for IHC next. I am interested in examining liver lung as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
S. Johnson
Verified customer
Asked: 2013-12-31
Answer
I took a look at the website and datasheets of our anti-Ubiquitin/UBB antibody and it seems that PA1420 has been validated on rat in both WB and IHC. Thus PA1420 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-12-31
