Product Info Summary
SKU: | A02848 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ubiquitin (K63-linkage Specific) UBB Antibody
SKU/Catalog Number
A02848
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Ubiquitin (K63-linkage Specific) UBB Antibody catalog # A02848. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ubiquitin (K63-linkage Specific) UBB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02848)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.738~750 (A-A-E-T-L) derived Human from PSMA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02848 is reactive to UBB in Human, Mouse, Rat
Applications
A02848 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
25.762kDa
Background of UBB
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4and isoform PSM-5would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000
ICC: 1:100-1:500
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of UBB using Anti-Ubiquitin (K63-linkage Specific) UBB Antibody (A02848).
ICC staining K63-linkage Specific Ubiquitin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Ubiquitin (K63-linkage Specific) UBB Antibody A02848
Western blot analysis of K63-linkage Specific Ubiquitin on Hela cells lysates using anti-K63-linkage Specific Ubiquitin antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For UBB (Source: Uniprot.org, NCBI)
Gene Name
UBB
Full Name
Polyubiquitin-B
Weight
25.762kDa
Superfamily
ubiquitin family
Alternative Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin UBB HEL-S-50 ubiquitin B polyubiquitin-B|epididymis secretory protein Li 50|polyubiquitin B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBB, check out the UBB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ubiquitin (K63-linkage Specific) UBB Antibody (A02848)
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No publications found for A02848
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