Anti-VAMP2 Antibody

Product Name

Anti-VAMP2 Antibody

View all VAMP-2 Antibodies

SKU/Catalog Number

A02331

Size

200μg/vial

Form

Liquid

Description

Boster Bio Anti-VAMP2 Antibody (Catalog# A02331). Tested in ICC, IP, WB application(s). This antibody reacts with Human, Mouse, Rat, Bovine, Guinea pig, Rabbit.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VAMP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02331)

Host

Rabbit

Contents

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic peptide corresponding to a portion of rat VAMP2. The sequence is completely conserved in mouse, human, bovine, and Xenopus

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A02331 is reactive to VAMP2 in Bovine, Guinea pig, Human, Mouse, Rabbit, Rat

Applications

A02331 is guaranteed for IP, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

12.663kDa

Background of VAMP-2

SOD3 (SUPEROXIDE DISMUTASE 3) also called SUPEROXIDE DISMUTASE, EXTRACELLULAR, EC-SOD, and Cu-Zn, is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Hendrickson et al. (1990) mapped the SOD3 gene to 4pter-q21 by a study of somatic cell hybrids. Stern et al. (2003) narrowed the assignment to 4p15.3-p15.1 by somatic cell and radiation hybrid analysis, linkage mapping, and FISH. The product of this gene is though to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1:1,000, ECL). Detects a band of ~18kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Western blot analysis of VAMP2 expression in MW Marker (lane 1), mouse brain extract (lane 2) and rat brain extract (lane 3). VAMP2 at 18KD was detected using rabbit anti-VAMP2 Antigen Affinity purified polyclonal antibody (Catalog # A02331) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For VAMP2 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ11460; SYB2; SYB2vesicle-associated membrane protein 2; synaptobrevin 2; synaptobrevin-2; VAMP2; VAMP-2; VAMP-2vesicle associated membrane protein 2; vesicle-associated membrane protein 2 (synaptobrevin 2) VAMP2 NEDHAHM, SYB2, VAMP-2 vesicle associated membrane protein 2 vesicle-associated membrane protein 2|synaptobrevin 2

For more info on VAMP2, check out the VAMP2 Infographic

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In this infographic, you will see the following information for VAMP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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