Anti-Vimentin Rabbit Monoclonal Antibody

Product Name

Anti-Vimentin Rabbit Monoclonal Antibody

View all Vimentin Antibodies

SKU/Catalog Number

M00235-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Vimentin Rabbit Monoclonal Antibody catalog # M00235-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Vimentin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00235-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AGF-22

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Vimentin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00235-1 is reactive to VIM in Human, Mouse, Rat

Applications

M00235-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

53.652kDa

Background of Vimentin

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:200-1:1000
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

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Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:1000 dilution.

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Figure 1. Western blot analysis of Vimentin using anti-Vimentin antibody (M00235-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (Catalog # M00235-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54 kDa. The expected band size for Vimentin is at 54 kDa.

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Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:1000 dilution.

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Immunohistochemical analysis of paraffin-embedded human colon, using Vimentin Antibody.

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Immunofluorescent analysis of Hela cells, using Vimentin Antibody.

Gene/Protein Information For VIM (Source: Uniprot.org, NCBI)

Alternative Names

FLJ36605; VIM; Vimentin VIM vimentin vimentin|epididymis secretory sperm binding protein

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