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Product Info Summary
| SKU: | M32320 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-VISTA VSIR Monoclonal Antibody [4C4]
View all VISTA/B7-H5/PD-1H Antibodies
SKU/Catalog Number
M32320
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-VISTA VSIR Monoclonal Antibody [4C4] (Catalog # M32320). Tested in ELISA, WB, IHC-P, ICC, IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
VISTA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-VISTA VSIR Monoclonal Antibody [4C4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M32320)
Host
Mouse
Contents
VISTA Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
Clone: 4C4
Isotype
IgG1
Immunogen
VISTA antibody was raised against the extracellular domain of human VISTA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
Reactive Species
M32320 is reactive to VSIR in Human
Applications
M32320 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
33.908kDa
Background of VISTA/B7-H5/PD-1H
VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VISTA antibody can be used for detection of VISTA by Western blot at 0.25 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of VISTA in overexpressing HEK293 cells with VISTA antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml
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Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
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Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 μg/mL.
Green: VISTA Antibody [4C4] (RF16071)
Blue: DAPI staining
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Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 5 μg/mL.
Red: VISTA Antibody [4C4] (RF16071)
Blue: DAPI staining
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Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 μg/mL.
Red: VISTA Antibody [4C4] (M32320)
Blue: DAPI staining
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Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 μg/mL.
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Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.
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Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.
Protein Target Info & Infographic
Gene/Protein Information For VSIR (Source: Uniprot.org, NCBI)
Gene Name
VSIR
Full Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
Weight
33.908kDa
Alternative Names
4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA; VSIR VSIR B7-H5, B7H5, C10orf54, DD1alpha, Dies1, GI24, PD-1H, PP2135, SISP1, VISTA V-set immunoregulatory receptor V-type immunoglobulin domain-containing suppressor of T-cell activation|Death Domain1alpha|PDCD1 homolog|V-domain Ig suppressor of T cell activation|V-set domain-containing immunoregulatory receptor|platelet receptor GI24|sisp-1|stress-induced secreted protein-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VSIR, check out the VSIR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VSIR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VISTA VSIR Monoclonal Antibody [4C4] (M32320)
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No publications found for M32320
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