Anti-Vitamin D Receptor VDR Rabbit Monoclonal Antibody

Product Name

Anti-Vitamin D Receptor VDR Rabbit Monoclonal Antibody

View all VDR/NR1I1/Vitamin D Receptor Antibodies

SKU/Catalog Number

M00210

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Vitamin D Receptor VDR Rabbit Monoclonal Antibody catalog # M00210. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Vitamin D Receptor VDR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00210)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAGE-22

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Vitamin D Receptor

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00210 is reactive to VDR in Human, Mouse, Rat

Applications

M00210 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

48 kDa

Calculated molecular weight

48.289kDa

Background of VDR/NR1I1/Vitamin D Receptor

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of VDR using anti-VDR antibody (M00210).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VDR antigen affinity purified monoclonal antibody (Catalog # M00210) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VDR at approximately 48 kDa. The expected band size for VDR is at 48 kDa.

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Gene/Protein Information For VDR (Source: Uniprot.org, NCBI)

Alternative Names

NR1I1; NR1I1Nuclear receptor subfamily 1 group I member 11,25-dihydroxyvitamin D3 receptor; VDR; vitamin D1,25- dihydroxyvitamin D3 receptor; vitamin D3 receptor VDR NR1I1, PPP1R163 vitamin D receptor vitamin D3 receptor|1,25-dihydroxyvitamin D3 receptor|nuclear receptor subfamily 1 group I member 1|protein phosphatase 1, regulatory subunit 163|vitamin D (1,25- dihydroxyvitamin D3) receptor|vitamin D nuclear receptor variant 1

For more info on VDR, check out the VDR Infographic

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In this infographic, you will see the following information for VDR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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