Anti-XRCC5 (phospho-T714) Antibody

Product Name

Anti-XRCC5 (phospho-T714) Antibody

View all Ku80/XRCC5 Antibodies

SKU/Catalog Number

A01275T714

Size

100ul

Form

Liquid

Description

Boster Bio Anti-XRCC5 (phospho-T714) Antibody catalog # A01275T714. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-XRCC5 (phospho-T714) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01275T714)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Tie-2 around the phosphorylation site of Y992.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01275T714 is reactive to XRCC5 in Human, Mouse, Rat

Applications

A01275T714 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

82.705kDa

Background of Ku80/XRCC5

Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress By similarity.

Delia J. Hern¨¢ndez-Deviez, Hum. Mol. Genet., Jan 2006; 15: 129 - 142.
Yanchao Huang, FASEB J, Mar 2007; 21: 732 - 742.
Mengfatt Ho, Hum. Mol. Genet., Sep 2004; 13: 1999 - 2010.
Eriko Fujita, Hum. Mol. Genet., Mar 2007; 16: 618 - 629.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of XRCC5 using Anti-XRCC5 (phospho-T714) Antibody (A01275T714).

Immunohistochemistry (IHC) analyzes of p-Ku-80 (T714) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
For more protocol information of IHC

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Figure 1. Western blotting validation for Anti-XRCC5 (phospho-T714) Antibody A01275T714

Western blot (WB) analysis of p-XRCC5 (T714) pAb at 1:500 dilution
Lane1:A549 whole cell lysate(40ug)
Lane2:A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate(40ug)
Lane3:A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug)
Lane4:The Brain tissue lysate of Mouse(40ug)
Lane5:The Uterus tissue lysate of Rat(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For XRCC5 (Source: Uniprot.org, NCBI)

Alternative Names

80kDa; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC85; CTCBF; EC 3.6.4.-; FLJ39089; G22P2; KARP1; KARP-1; Ku80; Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen; Ku86; Ku86DNA repair protein XRCC5; KUB2; NFIV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross-complementing XRCC5 KARP-1, KARP1, KU80, KUB2, Ku86, NFIV X-ray repair cross complementing 5 X-ray repair cross-complementing protein 5|86 kDa subunit of Ku antigen|ATP-dependent DNA helicase 2 subunit 2|ATP-dependent DNA helicase II 80 kDa subunit|CTC box-binding factor 85 kDa subunit|CTC85|CTCBF|DNA repair protein XRCC5|Ku autoantigen, 80kDa|Ku86 autoantigen related protein 1|TLAA|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)|lupus Ku autoantigen protein p86|nuclear factor IV|thyroid-lupus autoantigen

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