Anti-XRN2 Antibody Picoband™

Product Name

Anti-XRN2 Antibody Picoband™

View all XRN2 Antibodies

SKU/Catalog Number

A02797-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-XRN2 Antibody Picoband™ catalog # A02797-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-XRN2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02797-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human XRN2 recombinant protein (Position: A5-N748).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A02797-3 is reactive to XRN2 in Human

Applications

A02797-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

109 kDa

Calculated molecular weight

108.582kDa

Background of XRN2

5'-3' Exoribonuclease 2 (XRN2) also known as Dhm1-like protein is an exoribonuclease enzyme that in humans is encoded by the XRN2 gene. This gene encodes a 5'-3' exonuclease that promotes transcription termination at cotranscriptional cleavage sites. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of XRN2 using anti-XRN2 antibody (A02797-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human COLO-320 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human THP-1 whole cell lysates,
Lane 7: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRN2 antigen affinity purified polyclonal antibody (Catalog # A02797-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRN2 at approximately 109KD. The expected band size for XRN2 is at 109KD.

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Figure 2. Flow Cytometry analysis of A431 cells using anti-XRN2 antibody (A02797-3).
Overlay histogram showing A431 cells stained with A02797-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XRN2 Antibody (A02797-3, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For XRN2 (Source: Uniprot.org, NCBI)

Alternative Names

5'-3' exoribonuclease 2; Dhm1-like protein (mouse homolog); DHM1-like protein; DHP protein; EC 3.1.13; EC 3.1.13.- XRN2 5-3 exoribonuclease 2 5-3 exoribonuclease 2|DHM1-like protein|DHP protein

For more info on XRN2, check out the XRN2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for XRN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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