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- Table of Contents
BIK · Western blot design guide
BIK Western Blot Planning Guide
Plan a BIK Western blot around the catalog-observed 18 kDa band, image-backed PB9755 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
BIK Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 18 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 15% | |
| Positive control | Spleen | |
| Negative control | Cerebral cortex |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 18 kDa |
| ⓘ | Localization | Endomembrane system / Mitochondrion membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated BIK Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Gallbladder |
| Gel % | 15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.2 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9755 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected BIK Western Blot Band Size?
Use the product-observed 18 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 18 kDa | Matches the authoritative product WB observation. |
| 18 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected BIK appearancePlan around 18 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 18 kDa; use this as the primary experimental expectation. |
| Calculated mass | 18 kDa from UniProt Q13323; retain as context. |
| Gel selection | 15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9755. |
| Situation | Likely cause | Next action |
|---|---|---|
| 18 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing BIK band
PB9755 · BIK
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for BIK Western blot
🧪Use Spleen as the first positive-control candidate and Cerebral cortex as the HPA Not detected negative candidate.
Positive control: Spleen (High)
Negative control: Cerebral cortex (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for BIK
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Spleen | Reported tissue cells | High | Protein (HPA) | HPA → |
| Gallbladder | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Bone marrow | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced BIK Western Blot Tips
Deeper troubleshooting and optimisation questions for BIK, answered from its protein features.
Which band should guide the blot?
Use 18 kDa, the observation attached to the authoritative PB9755 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 18 kDa expectation.
Which positive control should I start with?
Start with Spleen, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Cerebral cortex as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for BIK Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9755 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
BIK Western Blot Reagents
Human/Mouse/Rat-reactive BIK Western blot reagents with authoritative product imagery.
Real WB data
Anti-Bik Antibody Picoband®
Cat # PB9755
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q13323
- Human Protein Atlas — BIK
- PB9755 product record
- PMC6719446 — BIK ubiquitination by the E3 ligase Cul5-ASB11 determines cell fate during cellular stress (The Journal of cell biology, 2019)
- PMC12280918 — BIK polymorphism and proteasome regulation unveil host risk factor for severe influenza (Proceedings of the National Academy of Sciences of the United States of America, 2025)
- PMC7127593 — NS5B induces up-regulation of the BH3-only protein, BIK, essential for the hepatitis C virus RNA replication and viral release (Virology, 2015)