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- Table of Contents
Designing a Western blot for MIF? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the MIF Western Blot GuideFacts about Macrophage migration inhibitory factor.
The expression of MIF in sites of inflammation suggests a role as mediator in regulating the use of macrophages in host defense. Counteracts the anti- inflammatory action of glucocorticoids.
| Human | |
|---|---|
| Gene Name: | MIF |
| Uniprot: | P14174 |
| Entrez: | 4282 |

| Belongs to: |
|---|
| MIF family |

EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase
Mass (kDA):
12.476 kDA

| Human | |
|---|---|
| Location: | 22q11.23 |
| Sequence: | 22; NC_000022.11 (23894383..23895223) |
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.





PMID: 2552447 by Weiser W.Y., et al. Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor.
PMID: 8234256 by Mikayama T., et al. Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor.
*More publications can be found for each product on its corresponding product page