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Product Info Summary
| SKU: | PROTP42574 |
|---|---|
| Size: | Starting from 10ug |
| Source: | Baculovirus |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
Human Caspase-3 Recombinant Protein
View all CASP3 recombinant proteins
SKU/Catalog Number
PROTP42574
Size
Starting from 10ug
Tag
His-Tag
Description
Human Caspase-3 Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 29-277aa. Application(s): SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.）
Cite This Product
Human Caspase-3 Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP42574)
Form
Liquid
Formulation
20mM HEPES buffer (pH 7.4) containing 0.1M NaCl, 1mM EDTA, 1mM DTT, 20% glycerol
Concentration
0.5mg/ml (determined by absorbance at 280nm)
Purity
> 90% by SDS-PAGE
Predicted MW
31.6 kDa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Amino Acid Sequence
Human, P42574, 29-277aa; MSGISLDNSY KMDYPEMGLC IIINNKNFHK STGMTSRSGT DVDAANLRET FRNLKYEVRN KNDLTREEIV ELMRDVSKED HSKRSSFVCV LLSHGEEGII FGTNGPVDLK KITNFFRGDR CRSLTGKPKL FIIQACRGTE LDCGIETDSG VDDDMACHKI PVEADFLYAY STAPGYYSWR NSKDGSWFIQ SLCAMLKQYA DKLEFMHILT RVNRKVATEF ESFSFDATFH AKKQIPCIVS MLTKELYFYHBioactivity
Specific activity is > 5,000 pmol/min/ug. One unit will liberate 1 pmole of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25°C.Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
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