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Product Info Summary
| SKU: | EK0722 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human CXCL1/Gro Alpha ELISA Kit PicoKine®
View all CXCL1/GRO alpha/KC/CINC-1 ELISA kits
SKU/Catalog Number
EK0722
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CXCL1/Gro Alpha ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CXCL1/Gro Alpha ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0722)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A35-N107
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0722 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0722 is reactive to CXCL1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0722 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL1/GRO alpha/KC/CINC-1
Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0722-CAP | Anti-Human CXCL1 Pre-coated 96-well strip microplate | 1 |
| EK0722-ST | Human CXCL1 Standard | 2 vials, 10 ng/tube |
| EK0722-DA | Human CXCL1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.044 | 0.098 | 0.203 | 0.309 | 0.498 | 0.877 | 1.335 | 1.967 |
Data Example Images
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Human CXCL1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CXCL1/GRO alpha/KC/CINC-1, Dilution ratio of 1:1, concentration in serum and plasma is around 200 pg/ml..
Some articles we found to cite concentrations of CXCL1/GRO alpha/KC/CINC-1 in samples: 7561066 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CXCL1/Gro Alpha ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 34 | 194 | 451 | 36 | 204 | 438 |
| Standard deviation | 2.44 | 9.7 | 29.31 | 2.95 | 10.81 | 36.51 |
| CV (%) | 7.2% | 5% | 6.5% | 8.2% | 5.3% | 8.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL1/GRO alpha/KC/CINC-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 35 | 39 | 33 | 35 | 2.27 | 6.4% |
| Sample 2 | 194 | 234 | 212 | 219 | 214 | 14.37 | 6.7% |
| Sample 3 | 451 | 412 | 454 | 480 | 449 | 24.28 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For CXCL1 (Source: Uniprot.Org, NCBI)
Gene Name
CXCL1
Full Name
Growth-regulated alpha protein
Weight
11.301kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha CXCL1 FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1 C-X-C motif chemokine ligand 1 growth-regulated alpha protein|C-X-C motif chemokine 1|GRO-alpha(1-73)|GRO1 oncogene (melanoma growth stimulating activity, alpha)|GRO1 oncogene (melanoma growth-stimulating activity)|MGSA alpha|chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)|fibroblast secretory protein|melanoma growth stimulating activity, alpha|melanoma growth stimulatory activity alpha|neutrophil-activating protein 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CXCL1, check out the CXCL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CXCL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CXCL1/Gro Alpha ELISA Kit PicoKine® (EK0722)
Hello CJ!
EK0722 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
RETRACTED ARTICLE: Sodium ferulate inhibits high-fat diet-induced inflammatory factors expression in human umbilical vein endothelial cells
Identification and Validation of Gene Expression Patterns in Cystitis Glandularis Patients and Controls:
Tumor cells educate mesenchymal stromal cells to release chemoprotective and immunomodulatory factors
Preclinical development of GR1501, a human monoclonal antibody that neutralizes interleukin-17A
Sodium ferulate inhibits high-fat diet-induced inflammatory factors expression in human umbilical vein endothelial cells
IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel
Species: Human
Interleukin-17 receptor D constitutes an alternative receptor for interleukin-17A important in psoriasis-like skin inflammation
Species: Human
XiaXia Man,XiaoLin Yang,ZhenTong Wei et al.High-level expression of CXCL1/GROα in uterine cervix cancer is linked to advanced stage and facilitates tumor cell malignant processes in an autocrine/paracrine manner, 06 May 2020, PREPRINT (Version 1) availabl
Species: Human
EK0722 usage in article: APP:ELISA, SAMPLE:HELA CELL AND PHM1-41 CELL, DILUTION:NA
IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel
Kong J, Kong L, Kong J, Ke S, Gao J, Ding X, Zheng L, Sun H, Sun W. J Transl Med. 2012 Nov 21;10:230. Doi: 10.1186/1479-5876-10-230. After Insufficient Radiofrequency Ablation, Tumor-Associated Endothelial Cells Exhibit Enhanced Angiogenesis And P...
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
10 Customer Q&As for Human CXCL1/Gro Alpha ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for CXCL1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-11-18
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CXCL1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-11-18
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
G. Garcia
Verified customer
Asked: 2020-10-25
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-10-25
Question
Q: What is the optimal O.D. value for CXCL1 ELISA kit? I performed your CXCL1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CXCL1 even though the O.D. values are not very high?
Z. Jha
Verified customer
Asked: 2020-08-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CXCL1 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of CXCL1 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2020-08-18
Question
Q: how to analyze ELISA data? I measured CXCL1 level in serum.
M. Allen
Verified customer
Asked: 2020-08-06
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-08-06
Question
Q: can you recommend the dilution ratio of serum samples for detection of CXCL1 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CXCL1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-01-08
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-03-21
Question
I have a customer who wants to order 50 kits of EK0722 now and another 450 as long as they test well. I need to know the turnaround on the first 50 kits and estimated production time for the other 450. Also both the 50 and the 450 need to be from the same lot. If there is a timeframe they need to order the 50 and then the 450 to keep the lot. Example within 2 months?
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
It takes one week for our lab technicains to prepare 50 kits of EK0722. If the customer need another 450 kits from the same lot, please notify us 5 weeks in advance. And we will send the 450 kits out 5 weeks later after we receive their notification. We can hold 450 kits for them for up to 6 months. Please let me know if you have any other questions.
Boster Scientific Support
Answered: 2017-08-21
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-12-20
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-12-20
Question
Q: if the enzyme conjugated CXCL1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXCL1 antigen?
Verified Customer
Verified customer
Asked: 2016-11-24
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-11-24
Question
Q: can I use heparin plasma as samples in Human CXCL1 Picokine® ELISA Kit (Catalog # EK0722)?
Verified Customer
Verified customer
Asked: 2016-01-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CXCL1 causing degradation of its protein structure. CXCL1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CXCL1 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-01-28
