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Product Info Summary
| SKU: | EK0678 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. |
Product info
Product Name
Human Cystatin C/CST3 ELISA Kit PicoKine®
View all Cystatin C ELISA kits
SKU/Catalog Number
EK0678
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Cystatin C/CST3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CST3 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Cystatin C/CST3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0678)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: M1-A146
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0678 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0678 is reactive to CST3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
Application Guarantee
EK0678 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cystatin C
Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0678-CAP | Anti-Human CST3 Pre-coated 96-well strip microplate | 1 |
| EK0678-ST | Human CST3 Standard | 2 vials, 20 ng/tube |
| EK0678-DA | Human CST3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.036 | 0.095 | 0.185 | 0.290 | 0.581 | 0.952 | 1.350 | 1.923 |
Data Example Images
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Human Cystatin C PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cystatin C, Dilution ratio of 1:500, concentration in serum and plasma is around 1500 ng/ml..
Some articles we found to cite concentrations of Cystatin C in samples: 24963810, 25216761, 24821156, 24661756, 22120484 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Cystatin C/CST3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 687 | 2837 | 10374 | 647 | 2715 | 10112 |
| Standard deviation | 43.96 | 207.1 | 684.68 | 51.11 | 217.2 | 859.52 |
| CV (%) | 6.4% | 7.3% | 6.6% | 7.9% | 8% | 8.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cystatin C in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 687 | 650 | 665 | 676 | 669 | 13.68 | 2% |
| Sample 2 | 2837 | 2995 | 2880 | 2796 | 2877 | 74.32 | 2.5% |
| Sample 3 | 10374 | 8987 | 8970 | 10046 | 9594 | 626.6 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For CST3 (Source: Uniprot.Org, NCBI)
Gene Name
CST3
Full Name
Cystatin-C
Weight
15.799kDa
Superfamily
cystatin family
Alternative Names
ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin CST3 ARMD11, HEL-S-2 cystatin C cystatin-C|bA218C14.4 (cystatin C)|cystatin 3|epididymis secretory protein Li 2|gamma-trace|neuroendocrine basic polypeptide|post-gamma-globulin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CST3, check out the CST3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CST3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Cystatin C/CST3 ELISA Kit PicoKine® (EK0678)
Hello CJ!
EK0678 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Elevated Urinary Levels of Cystatin C and Neutrophil Gelatinase-Associated Lipocalin in Henoch-Schönlein Purpura Patients with Renal Involvement
The value of plasma pro-enkephalin and adrenomedullin for the prediction of sepsis-associated acute kidney injury in critically ill patients
Prognostic impact of established and novel renal function biomarkers in myocardial infarction with cardiogenic shock: A biomarker substudy of the IABP-SHOCK II-trial
Effects of Therapeutic Ultrasound Applied to the Lumbar Region on Renal Function
Li, Z.,& Li, Z.(2021).Expression and significance of S‑100β, CysC and NF‑κB in patients with acute cerebral infarction.Experimental and Therapeutic Medicine,21,149.https://doi.org/10.3892/etm.2020.9580
Species: Human
EK0678 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Effects of Therapeutic Ultrasound Applied to the Lumbar Region on Renal Function: A Randomized Controlled Prospective Trial
Elevated Urinary Levels of Cystatin C and Neutrophil Gelatinase-Associated Lipocalin in Henoch-Sch?nlein Purpura Patients with Renal Involvement
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
11 Customer Q&As for Human Cystatin C/CST3 ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for Cystatin C ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-11-13
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Cystatin C for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-11-13
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
T. Parker
Verified customer
Asked: 2020-10-29
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-10-29
Question
I have a customer who wants to know why there is no wash step after step 5 -
Verified customer
Asked: 2020-09-24
Answer
There is no wash step after step 5. Our lab found there wasn't any significant difference in result values between washing and without washing the plate after incubating samples/standard and before addition of the detection antibody. It is to make the protocol easy and fast. It is OK if the customer wants to add washing step here. There won't be any harm.
Boster Scientific Support
Answered: 2020-09-25
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-02-21
Question
Are you able to provide a proprietary Certificate of EK0678?
Verified customer
Asked: 2019-08-18
Answer
We are uncertain if the Human Cystatin C ELISA Kit PicoKine™ (EK0678) is proprietary. Other manufacturer might develop and produce Human Cystatin C Elisa kit using the same principle and system as well. We can only guarantee that EK0678 is fully designed and developed by our company. The installations of the system are also performed by our qualified engineer.
Boster Scientific Support
Answered: 2019-08-19
Question
Q: can I use citrate plasma as samples in Human Cystatin C Picokine® ELISA Kit (Catalog # EK0678)?
Verified Customer
Verified customer
Asked: 2019-05-05
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Cystatin C causing disruption of its protein structure. Cystatin C may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Cystatin C ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-05-05
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Cystatin C using the EK0678 Human Cystatin C Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-11-27
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-11-27
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-09-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-09-11
Question
Q: Can Cystatin C ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-01-02
Question
I purchased the product EK0678 to use it in urine sample, if I want to store urine samples at -20, for how long it can be stored?
Verified Customer
Verified customer
Asked: 2017-02-22
Answer
The urine samples must be centrifuged at 10,000 x g at 4C for 2 minutes and the supernatant can either be used immediately or aliquoted and stored at -20 or -80C. However, we have not done a stability study over time to define exactly how long the Cystatin C will be stable under these conditions.
Boster Scientific Support
Answered: 2017-02-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
S. Campbell
Verified customer
Asked: 2016-12-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-12-05
